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    human-tau-elisa-kit-ab210972.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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SimpleStep

Human Tau ELISA Kit (ab210972)

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  • SDS
  • Protocol Booklet
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Other - Human Tau ELISA Kit (ab210972)
  • Example of human Tau standard curve in Sample Diluent NS.
  • Example of human Tau standard curve in 1X Extraction Buffer PTR.
  • Interpolated concentrations of spiked Tau in human serum, plasma, and cerebrospinal fluid samples.
  • Interpolated concentrations of native Tau in SH-SY5Y cell extract, human brain extract and human fetal brain extract samples based on a 1,000 µg/mL extract load.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.3 pg/ml
  • Range: 31.25 pg/ml - 2000 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Cow, Human

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Overview

  • Product name

    Human Tau ELISA Kit
    See all Tau kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Brain ext 5 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Brain ext 3 4.8%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.3 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 116 116% - 117%
    Cell culture extracts 111 107% - 117%
    Tissue Extracts 106 103% - 108%
    Hep Plasma 114 113% - 116%
    EDTA Plasma 107 105% - 109%
    Cerebral Spinal Fluid 103 101% - 104%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Cow, Human
  • Product overview

    Abcam is committed to meeting high quality standards of ethical manufacturing and has decided to discontinue this product because it contains antibodies generated by the ascites method. We are sorry for the inconvenience this may cause. This version will be discontinued when inventory is depleted. The new verison is available as ab273617.


    Human Tau ELISA Kit (ab210972) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tau protein in cell culture extracts, cell culture supernatant, cerebral spinal fluid, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Tau with 3.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Tau proteins constitute six isoforms from a single transcript from the MAPT gene that range from 48-68 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems, and are implicated in the assistance of microtubule assembly and stability. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems.

    The protein standard for the kit is Tau-F, and the kit can detect Tau-A. Other isoforms have not been detected.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Tau Capture Antibody 1 x 600µl
    10X Human Tau Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human Tau Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
  • Function

    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • Tissue specificity

    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
  • Involvement in disease

    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
  • Sequence similarities

    Contains 4 Tau/MAP repeats.
  • Developmental stage

    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
  • Domain

    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • Post-translational
    modifications

    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Target information above from: UniProt accession P10636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • AI413597
    • AW045860
    • DDPAC
    • FLJ31424
    • FTDP 17
    • G protein beta1/gamma2 subunit interacting factor 1
    • MAPT
    • MAPTL
    • MGC134287
    • MGC138549
    • MGC156663
    • Microtubule associated protein tau
    • Microtubule associated protein tau isoform 4
    • Microtubule-associated protein tau
    • MSTD
    • Mtapt
    • MTBT1
    • MTBT2
    • Neurofibrillary tangle protein
    • Paired helical filament tau
    • Paired helical filament-tau
    • PHF tau
    • PHF-tau
    • PPND
    • PPP1R103
    • Protein phosphatase 1, regulatory subunit 103
    • pTau
    • RNPTAU
    • TAU
    • TAU_HUMAN
    • Tauopathy and respiratory failure
    • Tauopathy and respiratory failure, included
    see all
  • Database links

    • Entrez Gene: 281296 Cow
    • Entrez Gene: 4137 Human
    • Omim: 157140 Human
    • SwissProt: P29172 Cow
    • SwissProt: P10636 Human
    • Unigene: 34217 Cow
    • Unigene: 101174 Human

    Associated products

      Images

      • Other - Human Tau ELISA Kit (ab210972)
        Other - Human Tau ELISA Kit (ab210972)

        SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

         

      • Example of human Tau standard curve in Sample Diluent NS.
        Example of human Tau standard curve in Sample Diluent NS.

        Background-subtracted data values (mean +/- SD) are graphed.

      • Example of human Tau standard curve in 1X Extraction Buffer PTR.
        Example of human Tau standard curve in 1X Extraction Buffer PTR.

        Background-subtracted data values (mean +/- SD) are graphed.

      • Interpolated concentrations of spiked Tau in human serum, plasma, and cerebrospinal fluid samples.
        Interpolated concentrations of spiked Tau in human serum, plasma, and cerebrospinal fluid samples.

        The concentrations of Tau were measured in duplicates, interpolated from the Tau standard curves and corrected for sample dilution. Undiluted samples are as follows:  serum 18.75%, plasma (EDTA) 18.75%, plasma (heparin) 18.75%, and cerebrospinal fluid 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

      • Interpolated concentrations of native Tau in SH-SY5Y cell extract, human brain extract and human fetal brain extract samples based on a 1,000 µg/mL extract load.
        Interpolated concentrations of native Tau in SH-SY5Y cell extract, human brain extract and human fetal brain extract samples based on a 1,000 µg/mL extract load.

        The concentrations of Tau were measured in duplicate and interpolated from the Tau standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean Tau concentration was determined to be 14.91 ng/mg in SH-SY5Y cell extract, 14.88 µg/mg in human brain extract and 92.05 µg/mg in human fetal brain extract.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab210972? Please let us know so that we can cite the reference in this datasheet.

      ab210972 has not yet been referenced specifically in any publications.

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