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  1. Link

    human-tau-unmodified--peptide-ab23425.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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Human Tau (unmodified ) peptide (ab23425)

  • Datasheet
Submit a review Q&A (1)

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ELISA - Human Tau (unmodified ) peptide (ab23425)

    Key features and details

    • Purity: > 90% HPLC
    • Suitable for: Blocking

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    Description

    • Product name

      Human Tau (unmodified ) peptide
      See all Tau proteins and peptides
    • Purity

      > 90 % HPLC.

    • Accession

      P10636
    • Animal free

      No
    • Nature

      Synthetic
      • Species

        Human

    Associated products

    • Immunizing Peptide (Blocking)

      • Human Tau (phospho S214) peptide (ab19123)

    Specifications

    Our Abpromise guarantee covers the use of ab23425 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C.

      Information available upon request.

    General Info

    • Alternative names

      • AI413597
      • AW045860
      • DDPAC
      • FLJ31424
      • FTDP 17
      • G protein beta1/gamma2 subunit interacting factor 1
      • MAPT
      • MAPTL
      • MGC134287
      • MGC138549
      • MGC156663
      • Microtubule associated protein tau
      • Microtubule associated protein tau isoform 4
      • Microtubule-associated protein tau
      • MSTD
      • Mtapt
      • MTBT1
      • MTBT2
      • Neurofibrillary tangle protein
      • Paired helical filament tau
      • Paired helical filament-tau
      • PHF tau
      • PHF-tau
      • PPND
      • PPP1R103
      • Protein phosphatase 1, regulatory subunit 103
      • pTau
      • RNPTAU
      • TAU
      • TAU_HUMAN
      • Tauopathy and respiratory failure
      • Tauopathy and respiratory failure, included
      see all
    • Function

      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
    • Tissue specificity

      Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
    • Involvement in disease

      Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
      Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
      Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
      Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
      Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
    • Sequence similarities

      Contains 4 Tau/MAP repeats.
    • Developmental stage

      Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
    • Domain

      The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
    • Post-translational
      modifications

      Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
      Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
      Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
    • Cellular localization

      Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
    • Target information above from: UniProt accession P10636 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • ELISA - Human Tau (unmodified ) peptide (ab23425)
      ELISA - Human Tau (unmodified ) peptide (ab23425)
      ab10891 gave a positive result in ELISA against the immunising peptide (ab19123). This ELISA shows that ab10891 antibody has some cross-reactivity with the non-modified equivalent peptide (ab23425) when used at high concentrations. Not yet tested in other applications.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab23425? Please let us know so that we can cite the reference in this datasheet.

    ab23425 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I was looking at the product Tau peptide - unmodified S214 (ab23425) . I have a few questions about the data sheet. It says that the length of protein is 757 amino acids which is larger than any tau isoform. Does this have tag? Are other sites phoshprlated as well? https://www.abcam.com/Tau-peptide-unmodified-S214-ab23425.html I had a hard time reading ELISA data. What concentration did you use in the ELISA? What do green and red lines stand for? Why is there increasing signal in higher concentration?

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2012

    Answer

    Thank you for contacting us.

    The peptide sequence is 18 amino acids long and it is notmodified on any other sites. The 757 amino acid length is generic and was set by the UniProt target IDP10636.

    I am still working to locate the source of this image, but we believe the image can be interpreted as follows:

    We suspect where they have stated concentration in the graph and in the legend, they meant to state dilution and so we are therefore not seeing increasing signal with higher concentration but decreasing signal at lower dilution i.e. the 1000 on the x-axis is 1/1000 dilution and the 100000 is 1/100000 dilution etc


    Based on dilution rather than concentration and from what is stated in the legend we believe the red line is peptide ab19123 (modified) and the green line is peptide ab23425 (non modified).

    I am checking which of our collaborators has submitted this image, and hopefully we will be able to further clarify the information on our datasheet. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on May 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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