Overview

  • Product name

    Human TCblR ELISA Kit (8D6A)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.1ng/ml 0.042 3.8%
    2 16 3.2ng/ml 0.14 4.5%
    3 16 6.3µg/ml 0.31 4.9%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.3ng/ml 0.08 6.2%
    2 24 3.4ng/ml 0.194 5.7%
    3 24 5.9ng/ml 0.313 5.3%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human TCblR Enzyme-Linked Immunosorbent Assay (ELISA) kit (8D6A) (ab213759) is designed for the quantitative measurement of Human TCblR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for TCblR has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TCblR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TCblR amount of sample captured in plate.

  • Notes

    TCblR (cluster of differentiation 320), also known as 8D6A or CD320, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and the expression of TCblR enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the TCblR signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. TCblR is also a specific receptor for uptake of TCN2-bound cobalamin.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human TCblR coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human TCblR antibody 1 x 130µl
    Lyophilized recombinant Human TCblR standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin.
  • Tissue specificity

    Expressed abundantly on follicular dendritic cells (FDCs).
  • Involvement in disease

    Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC) [MIM:613646]; also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.
  • Sequence similarities

    Contains 2 LDL-receptor class A domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • 8D6
    • 8D6 antigen
    • 8D6A
    • CD320
    • CD320 antigen
    • CD320_HUMAN
    • FDC-signaling molecule 8D6
    • FDC-SM-8D6
    • TCblR
    • Transcobalamin receptor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human TCblR ELISA Kit (8D6A) (ab213759) Standard Curve.

Protocols

References

ab213759 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213759.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up