Human TDP43 ELISA Kit (TARDBP) (ab282880)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 248.24 pg/ml
- Range: 1171.87 pg/ml - 75000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TDP43 ELISA Kit (TARDBP)
See all TDP43 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 6.2% Inter-assay Sample n Mean SD CV% Extract 3 7.8% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
248.24 pg/ml -
Range
1171.87 pg/ml - 75000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 111 108% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TDR43 ELISA kit (ab282880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TDR43 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TDR43 with 248.24 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human TDP43 is an RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, human TDP43 regulates the splicing of many non-coding and protein coding RNAs including proteins involved in neuronal survival and mRNAs that encode proteins relevant for neurodegenerative diseases. Human TDP43 plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Human TDP43 regulates mRNA. It also participates in normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. It is involved with regulation of expression of CDK6 and HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TDP43 (TARDBP) Capture Antibody 1 x 600µl 10X Human TDP43 (TARDBP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human TDP43 (TARDBP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. -
Tissue specificity
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. -
Involvement in disease
Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The RRM domains can bind to both DNA and RNA. -
Post-translational
modificationsHyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. -
Cellular localization
Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies. - Information by UniProt
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Alternative names
- ALS10
- OTTHUMP00000002171
- OTTHUMP00000002172
see all -
Database links
- Entrez Gene: 23435 Human
- Omim: 605078 Human
- SwissProt: Q13148 Human
- Unigene: 300624 Human
- Unigene: 635053 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human TDP43 standard curve in 1X Cell Extraction Buffer PTR.The TDP43 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TDP43 in human K562 extract, OVCAR-3 extract, and human skeletal muscle extractInterpolated concentrations of native TDP43 in human K562 extract, OVCAR-3 extract, and human skeletal muscle extract based on a 20 μg/mL extract load for K562 extract, 50 μg/mL extract load for OVCAR-3 extract, and 100 μg/mL extract load for skeletal muscle extract. The concentrations of TDP43 were measured in duplicate and interpolated from the TDP43 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TDP43 concentration was determined to be 70.6 ng/mL in K562 extract, 89.24 ng/mL in OVCAR-3 extract and 85.05 ng/mL in skeletal muscle extract.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab282880 has not yet been referenced specifically in any publications.