For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    human-teck-elisa-kit-ab242251.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Chemokines Beta Chemokines (CC)
Share by email
RecombinantSimpleStep

Human TECK ELISA Kit (ab242251)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Other - Human TECK ELISA Kit (ab242251)
  • Example of human TECK standard curve in Sample Diluent NS.
  • Interpolated concentrations of spiked TECK in human serum, plasma and cell culture supernatant samples.
  • Sandwich ELISA - Human TECK ELISA Kit (ab242251)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.9 pg/ml
  • Range: 23.44 pg/ml - 1500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

Protein
Recombinant human CCL25 protein (ab586)

View more associated products

Overview

  • Product name

    Human TECK ELISA Kit
    See all CCL25 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 4.6%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 5.2%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.9 pg/ml
  • Range

    23.44 pg/ml - 1500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99 99% - 100%
    Serum 94 91% - 99%
    EDTA Plasma 98 93% - 100%
    Cit plasma 97 93% - 99%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human TECK ELISA Kit (ab242251) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TECK protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TECK with 1.9 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human TECK Capture Antibody 1 x 600µl
    10X Human TECK Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human TECK Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
  • Function

    Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • Tissue specificity

    Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O15444 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • A130072A22Rik
    • C-C motif chemokine 25
    • CCL 25
    • CCL25
    • CCL25_HUMAN
    • Chemokine (C C motif) ligand 25
    • Chemokine (CC motif) ligand 25
    • Chemokine C C motif ligand 25
    • Chemokine CC motif ligand 25
    • Chemokine TECK
    • Ck beta 15
    • Ckb 15
    • Ckb15
    • MGC125074
    • MGC150327
    • SCY A25
    • SCYA 25
    • SCYA25
    • Small inducible cytokine A25
    • Small inducible cytokine A25 isoform 1 precursor
    • Small inducible cytokine A25 isoform 2 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 25
    • Small inducible cytokine subfamily A Cys Cys member 25
    • Small inducible cytokine subfamily A, member 25
    • Small-inducible cytokine A25
    • TECK
    • TECKvar
    • Thymus expressed chemokine
    • Thymus-expressed chemokine
    see all
  • Database links

    • Entrez Gene: 6370 Human
    • Omim: 602565 Human
    • SwissProt: O15444 Human
    • Unigene: 310511 Human

    Associated products

    • Alternative Versions

      • Human TECK Antibody Pair - BSA and Azide free (ab256624)

    Images

    • Other - Human TECK ELISA Kit (ab242251)
      Other - Human TECK ELISA Kit (ab242251)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human TECK standard curve in Sample Diluent NS.
      Example of human TECK standard curve in Sample Diluent NS.
      The TECK standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of spiked TECK in human serum, plasma and cell culture supernatant samples.
      Interpolated concentrations of spiked TECK in human serum, plasma and cell culture supernatant samples.
      The concentrations of TECK were measured in duplicates, interpolated from the TECK standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 12.5%, and cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TECK concentration was determined to be 1007.84 pg/mL in serum, 1066.68 pg/mL in plasma (citrate), 1091.29 pg/mL in plasma (EDTA) and 916.63 pg/mL in cell culture supernatant.
    • Sandwich ELISA - Human TECK ELISA Kit (ab242251)
      Sandwich ELISA - Human TECK ELISA Kit (ab242251)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab242251? Please let us know so that we can cite the reference in this datasheet.

    ab242251 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab242251.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.