Human TECK ELISA Kit (ab242251)
- Datasheet
- References
- Protocols
Overview
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Product name
Human TECK ELISA Kit
See all CCL25 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4.6% Inter-assay Sample n Mean SD CV% serum 3 5.2% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.9 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 99 99% - 100% Serum 94 91% - 99% EDTA Plasma 98 93% - 100% Cit plasma 97 93% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
TECK in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TECK protein in human serum, plasma, and cell culture media.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Human TECK (CCL25) is a CC chemokine produced by stromal cells in the thymus and epithelial cells of the small intestine. TECK is involved in T-cell development inducing chemoattraction through CCR9 on immature pre-T cells and thymocytes. TECK induces the homing of several lymphocyte populations to the small intestine, including integrin a4b7+ gamma/delta T cells, Integrin aEb7+ CD8+ T cells, and IgA-producing plasma cells. In cancer, TECK promotes the metastasis of melanoma cells, migration and invasion of some breast carcinomas, and mesenchymal stromal cells in multiple myelomas. TECK attracts CCR9+ monocytes and macrophages in rheumatoid arthritis and CCR9+ macrophages in atherosclerosis. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils. Isoform 2 of TECK is an antagonist of isoform 1. Human TECK shares 45% and 43.7% sequence homology with mouse and rat, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TECK Capture Antibody 1 x 600µl 10X Human TECK Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human TECK Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. -
Tissue specificity
Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A130072A22Rik
- C-C motif chemokine 25
- CCL 25
see all -
Database links
- Entrez Gene: 6370 Human
- Omim: 602565 Human
- SwissProt: O15444 Human
- Unigene: 310511 Human
Associated products
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Alternative Versions
Images
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Other - Human TECK ELISA Kit (ab242251)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TECK standard curve in Sample Diluent NS.The TECK standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of spiked TECK in human serum, plasma and cell culture supernatant samples.The concentrations of TECK were measured in duplicates, interpolated from the TECK standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 12.5%, and cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TECK concentration was determined to be 1007.84 pg/mL in serum, 1066.68 pg/mL in plasma (citrate), 1091.29 pg/mL in plasma (EDTA) and 916.63 pg/mL in cell culture supernatant.
Protocols
References
ab242251 has not yet been referenced specifically in any publications.
