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|Sample type||Average %||Range|
|Cell culture supernatant||109.5||93% - 132%|
|Plasma||123.8||113% - 132%|
Abcam’s TECK Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TECK in plasma and cell culture supernatants. (Human TECK concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human TECK coated on a 96-well plate. Standards and samples are pipetted into the wells and TECK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TECK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TECK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human TECK in normal serum with ab100645, only in serum spiked with Human TECK.
Optimization may be requried with urine samples.
|Components||1 x 96 tests|
|1,000X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human TECK||2 vials|
|Recombinant Human TECK Standard (lyophilized)||2 vials|
|Stop Solution||1 x 25ml|
|TECK Microplate (12 x 8 wells)||1 unit|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100645 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"