Human TECK ELISA Kit (Thymus Expressed Chemokine) (ab100645)


  • Product name
    Human TECK ELISA Kit (Thymus Expressed Chemokine)
    See all TECK kits
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 200 pg/ml
  • Range
    0.206 ng/ml - 150 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 109.5 93% - 132%
    Plasma 123.8 113% - 132%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TECK Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TECK in plasma and cell culture supernatants. (Human TECK concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human TECK coated on a 96-well plate. Standards and samples are pipetted into the wells and TECK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TECK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TECK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Human TECK in normal serum with ab100645, only in serum spiked with Human TECK.

  • Notes

    Optimization may be requried with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    1,000X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TECK 2 vials
    Recombinant Human TECK Standard (lyophilized) 2 vials
    Stop Solution 1 x 25ml
    TECK Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
  • Alternative names
    • C C motif chemokine 25
    • CCL 25 MGC125074
    • CCL25
    • Chemokine (C C motif) ligand 25
    • Chemokine TECK
    • Ck beta 15
    • Ckb 15
    • Ckb15
    • MGC150327
    • SCY A25
    • SCYA 25
    • SCYA25
    • Small inducible cytokine A25
    • Small inducible cytokine A25 isoform 1 precursor
    • Small inducible cytokine A25 isoform 2 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 25
    • Small inducible cytokine subfamily A Cys Cys member 25
    • Small inducible cytokine subfamily A, member 25
    • TECKvar
    • Thymus expressed chemokine
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100645

  • Representative standard curve using ab100645



ab100645 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The recommended starting dilution for testing normal serum/plasma with the kit is 2-fold. However, please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the in...

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