Key features and details
- Sensitivity: 10 pg/ml
- Range: 93.7 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Tenascin-C ELISA Kit (TNC)
See all Tenascin C kits
Intra-assay Sample n Mean SD CV% 1 16 94pg/ml 4.51 = 4.8% 2 16 868pg/ml 61.62 = 7.1% 3 16 3019pg/ml 223.4 = 7.4% Inter-assay Sample n Mean SD CV% 1 24 89pg/ml 5.51 = 6.2% 2 24 892pg/ml 74.03 = 8.3% 3 24 3055pg/ml 226.07 = 7.4%
Sample typeCell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range93.7 pg/ml - 6000 pg/ml
Assay time3h 30m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
The Human Tenascin-C Enzyme-Linked Immunosorbent Assay (ELISA) kit (TNC) (ab213831) is designed for the quantitative measurement of Human Tenascin-C in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tenascin-C/TNC has been pre-coated onto 96-well plates. Standards (Expression system for standard (NSO; Immunogen sequence: G23-P625) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tenascin-C/TNC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tenascin-C/TNC amount of sample captured in plate.
Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Tenascin-C coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Tenascin-C antibody 1 x 100µl Lyophilized recombinant Human Tenascin-C standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionExtracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
Sequence similaritiesBelongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 150 225
- Glioma associated extracellular matrix antigen
ab213831 has been referenced in 1 publication.
- Pirklbauer M et al. Unraveling reno-protective effects of SGLT2 inhibition in human proximal tubular cells. Am J Physiol Renal Physiol 316:F449-F462 (2019). PubMed: 30539648