Overview

  • Product name

    Human Tenascin-C ELISA Kit (TNC)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 843pg/ml 43.8 5.2%
    2 16 2186pg/ml 118 5.4%
    3 16 3627pg/ml 192.7 5.9%
    Inter-assay
    Sample n Mean SD CV%
    1 24 924pg/ml 53.5 5.8%
    2 24 2570pg/ml 159.3 6.2%
    3 24 4085pg/ml 249.1 6.1%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    93.7 pg/ml - 6000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Tenascin-C Enzyme-Linked Immunosorbent Assay (ELISA) kit (TNC) (ab213831) is designed for the quantitative measurement of Human Tenascin-C in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tenascin-C/TNC has been pre-coated onto 96-well plates. Standards (Expression system for standard (NSO; Immunogen sequence: G23-P625) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tenascin-C/TNC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tenascin-C/TNC amount of sample captured in plate.

  • Notes

    Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Tenascin-C coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Tenascin-C antibody 1 x 130µl
    Lyophilized recombinant Human Tenascin-C standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
  • Sequence similarities

    Belongs to the tenascin family.
    Contains 15 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 15 fibronectin type-III domains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • 150 225
    • Cytotactin
    • Glioma associated extracellular matrix antigen
    • Glioma-associated-extracellular matrix antigen
    • GMEM
    • GP
    • GP 150 225
    • GP 150-225
    • Hexabrachion
    • HXB
    • JI
    • MGC167029
    • Miotendinous antigen
    • Myotendinous antigen
    • Neuronectin
    • TENA_HUMAN
    • Tenascin
    • Tenascin-C
    • Tenascin-C isoform 14/AD1/16
    • TenascinC
    • TN
    • TN C
    • TN-C
    • TNC
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Tenascin-C ELISA Kit (TNC) (ab213831) Standard Curve.

Protocols

References

ab213831 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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