Overview

  • Product name
    Human Testosterone free ELISA Kit
    See all Testosterone kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sera 15 < 10%
    Inter-assay
    Sample n Mean SD CV%
    Sera 10 < 10%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Competitive
  • Sensitivity
    >= 0.06 pg/ml
  • Range
    0.1 pg/ml - 42 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

    A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab178663.

Protocols

References

ab178663 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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