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    human-testosterone-free-elisa-kit-ab178663.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Testosterone
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Human Testosterone free ELISA Kit (ab178663)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.06 pg/ml
    • Range: 0.1 pg/ml - 42 pg/ml
    • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Human

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    Overview

    • Product name

      Human Testosterone free ELISA Kit
      See all Testosterone kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Sera 15 < 10%
      Inter-assay
      Sample n Mean SD CV%
      Sera 10 < 10%
    • Sample type

      Serum, Hep Plasma, EDTA Plasma, Cit plasma
    • Assay type

      Competitive
    • Sensitivity

      >= 0.06 pg/ml
    • Range

      0.1 pg/ml - 42 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.

      A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      10X Wash Solution 1 x 50ml
      Anti-Testosterone IgG Coated Microplate (12 x 8 wells) 1 unit
      Cover Foil 1 unit
      Free Testosterone Control A 1 x 1ml
      Free Testosterone Control B 1 x 1ml
      Stop Solution 1 x 15ml
      Strip holder 1 unit
      Testosterone HRP Conjugate 1 x 15ml
      Testosterone Standard 0 - 0 pg/mL 1 x 1ml
      Testosterone Standard 1 - 0.2 pg/mL 1 x 1ml
      Testosterone Standard 2 - 1.0 pg/mL 1 x 1ml
      Testosterone Standard 3 - 4.0 pg/mL 1 x 1ml
      Testosterone Standard 4 - 20 pg/mL 1 x 1ml
      Testosterone Standard 5 - 100 pg/mL 1 x 1ml
      TMB Substrate Solution 1 x 15ml
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Testosterone
      • Signal Transduction
      • Growth Factors/Hormones
      • Hormones
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Testosterone
      • Developmental Biology
      • Reproduction
      • Hormones
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Growth factors and hormones ELISA kits
    • Alternative names

      • 17ß-hydroxyandrost-4-ene-3-one
    • Database links

      • SwissProt: P04278 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative standard curve using ab178663.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab178663? Please let us know so that we can cite the reference in this datasheet.

        ab178663 has not yet been referenced specifically in any publications.

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