Human Testosterone free ELISA Kit (ab178663)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Testosterone free ELISA Kit
See all Testosterone kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sera 15 < 10% Inter-assay Sample n Mean SD CV% Sera 10 < 10% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
>= 0.06 pg/ml -
Range
0.1 pg/ml - 42 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Free Testosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti- Free Testosterone antibodies (solid-phase). Samples and the Free Testosterone-HRP conjugate are added to the wells, where Free Testosterone in the sample competes with the added Free Testosterone-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Free Testosterone in the sample and the intensity is measured at 450 nm.
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Tested applications
Suitable for: Competitive ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Testosterone IgG Coated Microplate (12 x 8 wells) 1 unit Cover Foil 1 unit Free Testosterone Control A 1 x 1ml Free Testosterone Control B 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit Testosterone HRP Conjugate 1 x 15ml Testosterone Standard 0 - 0 pg/mL 1 x 1ml Testosterone Standard 1 - 0.2 pg/mL 1 x 1ml Testosterone Standard 2 - 1.0 pg/mL 1 x 1ml Testosterone Standard 3 - 4.0 pg/mL 1 x 1ml Testosterone Standard 4 - 20 pg/mL 1 x 1ml Testosterone Standard 5 - 100 pg/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- 17ß-hydroxyandrost-4-ene-3-one
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Database links
- SwissProt: P04278 Human
Applications
Our Abpromise guarantee covers the use of ab178663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab178663.
Protocols
References
ab178663 has not yet been referenced specifically in any publications.
