Overview

  • Product name

    Human Tetranectin ELISA Kit (CLEC3B)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 2.3ng/ml 0.106 4.6%
    2 16 4.7ng/ml 0.174 3.7%
    3 16 9.8ng/ml 0.51 5.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 2.6ng/ml 0.143 5.5%
    2 24 5ng/ml 0.245 4.9%
    3 24 10.2ng/ml 0.643 6.3%
  • Sample type

    Cell culture supernatant, Serum, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    312 pg/ml - 20000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human Tetranectin Enzyme-Linked Immunosorbent Assay (ELISA) kit (CLEC3B) (ab213832) is designed for the quantitative measurement of Human Tetranectin in cell supernatants, serum and tissue homogenates.


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tetranectin/CLEC3B has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: E22-V202) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tetranectin/CLEC3B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tetranectin/CLEC3B amount of sample captured in plate.

  • Notes

    Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and non-covalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human Tetranectin coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human Tetranectin antibody 1 x 130µl
    Lyophilized recombinant Human Tetranectin standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
  • Tissue specificity

    Found in plasma.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C type lectin domain family 3 member B
    • C-type lectin domain family 3 member B
    • Clec3b
    • Plasminogen kringle 4 binding protein
    • Plasminogen kringle 4-binding protein
    • TETN_HUMAN
    • Tetranectin
    • Tetranectin (plasminogen binding protein)
    • TN
    • TNA
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Tetranectin ELISA Kit (CLEC3B) (ab213832) Standard Curve.

Protocols

References

ab213832 has not yet been referenced specifically in any publications.

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