Overview

  • Product name

    Human TFPI2 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.75ng/ml 0.074 4.2%
    2 16 3.28ng/ml 0.154 4.7%
    3 16 6.32ng/ml 0.354 5.6%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.64ng/ml 0.08 4.9%
    2 24 3.09ng/ml 0.164 5.3%
    3 24 6.47ng/ml 0.395 6.1%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human TFPI2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213836) is designed for the quantitative measurement of Human TFPI2 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFPI2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: D23-F235) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TFPI2 amount of sample captured in plate.

  • Notes

    TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human TFPI2 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human TFPI2 antibody 1 x 130µl
    Lyophilized recombinant Human TFPI2 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
  • Tissue specificity

    Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.
  • Sequence similarities

    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain

    This inhibitor contains three inhibitory domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Placental protein 5
    • PP5
    • REF1
    • Retinal pigment epithelium cell factor 1
    • TFPI 2
    • TFPI-2
    • TFPI2
    • TFPI2 PEN
    • TFPI2_HUMAN
    • Tissue factor pathway inhibitor 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human TFPI2 ELISA Kit (ab213836) Standard Curve.

Protocols

References

ab213836 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213836.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up