• Product name

    Human TGF alpha ELISA Kit
  • Detection method

  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 3 pg/ml
  • Range

    1.37 pg/ml - 1000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.49 84% - 105%
    Serum 92.34 82% - 104%
    Plasma 93.46 83% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TGF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF alpha in serum, plasma, cell culture supernatants

    This assay employs an antibody specific for Human TGF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TGF alpha 2 vials
    Recombinant Human TGF alpha Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TGF alpha Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Transforming growth factor alpha (TGF alpha) is an acid- and heat-stable originally discovered in rodents and humans. TGF alpha protien is 33% homologous to epidermal growth factor (EGF). TGF alpha binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF alpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells . TGF alpha bioactivity has also been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
  • Alternative names

    • EGF like TGF
    • ETGF
    • OTTHUMP00000203300
    • OTTHUMP00000203301
    • OTTHUMP00000203345
    • OTTHUMP00000203352
    • Protransforming growth factor alpha
    • TFGA
    • TGF A
    • TGF type 1
    • TGFA
    • Transforming growth factor alpha
    • Transforming growth factor alpha precursor
    • Wa1
    • Waved 1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100646

  • Representative standard curve using ab100646



This product has been referenced in:

  • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). Human . Read more (PubMed: 27141367) »
  • Mouradian M  et al. Key roles for GRB2-associated-binding protein 1, phosphatidylinositol-3-kinase, cyclooxygenase 2, prostaglandin E2 and transforming growth factor alpha in linoleic acid-induced upregulation of lung and breast cancer cell growth. Prostaglandins Leukot Essent Fatty Acids 90:105-15 (2014). Read more (PubMed: 24374147) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacellulin). The most cost effective approach for you might be an indirect ELISA, which requires coating the sample onto the plate, then detecting with an antibody specific for the protein of interest and then detecting that with an anti-IgG secondary antibody conjugated to an enzyme such as HRP. Please let me know if you have any questions.

Read More


Merci de nous avoir contactés.

Le kit ELISA ab100646 est en effet approprié pour le dosage du TGF-alpha humain contenu dans les supernageants de cultures cellulaires, urine, sérum et plasma.

Pour les anti-Histone 3 phosphorylé, j'ajouterais aux 3 références ab5176, ab47297 et ab4076, le polyclonal de lapin anti-Histone 3 (phospho T6) référence ab14102 (https://www.abcam.com/ab14102).
Je recommanderais également les 3 monoclonaux de lapin suivants:
Anti-Histone H3 (phospho S10) ab32107, https://www.abcam.com/ab32107
Anti-Histone H3 (phospho S28) ab32388, https://www.abcam.com/ab32388
Anti-Histone H3 (phospho T3) ab78351, https://www.abcam.com/ab78351

Pour les anti-Caspase 3 clivée, le ab52294 semble convenir. Malheureusement les nouveaux lots de cet anticorps ne passent pas pour l'instant les contrôles qualité et le délai de livraison risque d'être long (2 mois minimum).
Le ab52293 ne reconnait pas uniquement la forme clivée (fragment p17) mais également la forme non-clivée.
Je crains que nous n'ayons pas pour l'instant d'anticorps spécifique de la forme clivée de la Caspase 3.
Nous avons également pour la Caspase 3 un monoclonal de lapin, référence ab32351 qui reconnait les 2 formes (pro-forme et p17). Le lien vers la fiche technique est https://www.abcam.com/ab32351.

Parmi la liste des secondaires anti-lapin que nous avons en catalogue, voici la liste des produits que je recommanderais:
HRP, ab97064, https://www.abcam.com/ab97064
AP, ab97061, https://www.abcam.com/ab97061
FITC, ab98502, https://www.abcam.com/ab98502
Texas Red, ab6800, https://www.abcam.com/ab600
DyLight® 488, ab96891, https://www.abcam.com/ab96891
DyLight® 550, ab96892, https://www.abcam.com/ab96892
DyLight® 594, ab96893, https://www.abcam.com/ab96893
DyLight® 650, ab96894, https://www.abcam.com/ab96894

J'espère que ces informations vous seront utiles.

N'hésitez pas à me communiquer la liste des produits qui vous intéressent, je transmettrai alors cette liste à mes collègues du service marketing qui vous enverront un devis.

Read More

Abreview for TGF alpha Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Human Serum (serum)
Specification: serum
Amount of Sample: 20 uL µg
Type of Kit Used: ELISA
Positive control: We did not measure positive control except for kit standards.
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

Submitted Aug 03 2012

For licensing inquiries, please contact partnerships@abcam.com

Sign up