Overview

  • Product name

    Human TGF beta 1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.6%
  • Sample type

    Cell culture supernatant
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.03 ng/ml
  • Range

    0.031 ng/ml - 2 ng/ml
  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TGF beta 1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TGF beta 1 concentrations in cell culture supernatants.


    A TGF beta 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TGF beta 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TGF beta 1 captured in plate. This assay recognizes both natural and recombinant Human TGF beta 1.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 20ml
    15X Biotinylated Human TGF beta 1 Antibody 1 x 350µl
    20X Wash Buffer Concentrate 2 x 30ml
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Standard Diluent 1 x 2ml
    Stop Solution 1 x 12ml
    TGF beta 1 Microplate (12 x 8 well strips) 1 unit
    TGF beta 1 Standard 1 vial
  • Research areas

  • Function

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease

    Camurati-Engelmann disease
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Domain

    The 'straitjacket' and 'arm' domains encircle the growth factor monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. Activation of TGF-beta1 requires the binding of integrin alpha-V to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-beta binding proteins. The sheer physical force unfastens the straitjacket and releases the active growth factor dimer.
  • Post-translational
    modifications

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • LAP
    • Latency-associated peptide
    • TGF-beta-1
    • tgfb1
    • TGFB1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108912.

Protocols

References

This product has been referenced in:

  • Li W  et al. LncRNA POU3F3 promotes cancer cell migration and invasion in nasopharyngeal carcinoma by up-regulating TGF-ß1. Biosci Rep 39:N/A (2019). Read more (PubMed: 30602452) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer



Avec ab100647, nous recommandons d’activer vos échantillons en faisant une extraction à l'acide, voir page 9 -10 du protocole.

Il est fortement recommandé d’effectuer cette l'extraction à l'acide car la TGFb actif naturellement sécrétée par les cellules est très peu abondante dans des échantillons normaux.

Selon nos données sur des échantillons normaux de sérum humain, la concentration apparente de TGF bêta 1 sans activation était d'environ 100 pg / ml. La valeur de la DO était assez faible et proche de la valeur DO pour les échantillons blancs. Cependant, après extraction, nous avons eu environ 2000 pg / ml.

Ce kit peut détecter entre 0.082 ng/ml - 60 ng/ml de TGF beta 1 mais il semblerait que sans l’activation du TGFb, les valeurs seraient plutôt vers l'extrémité inférieure de cette gamme. Je vous recommanderais plutôt d’utiliser TGF beta 1 Human ELISA Kit (ab108912). Ce kit ne suggère aucune extraction à l'acide et à une meilleure sensibilité de 0.031 ng/ml - 2 ng/ml.

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Answer

Gracias por contactarnos de nuevo.

Me alegra saber que el kit os ha dado buenos resultados.

Si quieres tramitar el pedido de un nuevo kit, o para tratar temas de promociones y ofertas, por favor contacta con nuestro departamento de Servicio al Cliente en el 91 116 65 54 o bien escribiendo a mailto:orders@abcam.com.

Que tengas un buen día.

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Answer

Thank you for contacting Abcam. Please find the requested Certificate of Compliances attached. If there is anything else that I can help you with, please let me kn

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