Overview

  • Product name
    Human TGF beta R2 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.5 pg/ml
  • Range
    1.5 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97.91 90% - 106%
    Plasma 94.61 87% - 103%
    Cell culture media 121.2 99% - 133%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TGF beta R2 Human ELISA Kit (ab193715) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta R2 in serum, plasma and cell culture supernatant.

    This assay employs an antibody specific for Human TGF beta R2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TGF beta R2 present in the samples. The wells are washed and biotinylated anti-Human TGF beta R2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TGF beta R2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated Human TGF-beta R2 detection antibody (lyophilized) 2 vials
    Human TGF-beta R2 standards (lyophilized) 2 vials
    Pre-coated Human TGF-beta R2 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
  • Involvement in disease
    Hereditary non-polyposis colorectal cancer 6
    Esophageal cancer
    Loeys-Dietz syndrome 2
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on a Ser/Thr residue in the cytoplasmic domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Alternative names
    • AAT3
    • FAA3
    • LDS1B
    • LDS2
    • LDS2B
    • MFS2
    • RIIC
    • TAAD2
    • TbetaR-II
    • TGF-beta receptor type II
    • TGF-beta receptor type-2
    • TGF-beta type II receptor
    • TGFbeta RII
    • Tgfbr2
    • TGFR 2
    • TGFR-2
    • TGFR2_HUMAN
    • Transforming growth factor beta receptor II (70/80kDa)
    • Transforming growth factor-beta receptor type II
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve in Assay Diluent A using ab193715 TGF beta R2 Human ELISA Kit.

  • Typical Standard Curve in Assay Diluent B using ab193715 TGF beta R2 Human ELISA Kit.

Protocols

References

ab193715 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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