Abcam’s TGF beta R2 Human ELISA Kit (ab193715) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta R2 in serum, plasma and cell culture supernatant.
This assay employs an antibody specific for Human TGF beta R2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TGF beta R2 present in the samples. The wells are washed and biotinylated anti-Human TGF beta R2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TGF beta R2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Biotinylated Human TGF-beta R2 detection antibody (lyophilized)
Human TGF-beta R2 standards (lyophilized)
Pre-coated Human TGF-beta R2 Microplate (12 strips x 8 wells)
1 x 8ml
TMB One-Step Substrate Reagent
1 x 12ml
Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
Involvement in disease
Hereditary non-polyposis colorectal cancer 6 Esophageal cancer Loeys-Dietz syndrome 2
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 protein kinase domain.
Phosphorylated on a Ser/Thr residue in the cytoplasmic domain.