Overview

  • Product name

    Human TGFBR3 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.15ng/ml 0.051 4.5%
    2 16 2.52ng/ml 0.108 4.3%
    3 16 5.81ng/ml 0.284 4.9%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.63ng/ml 0.109 6.7%
    2 24 2.87ng/ml 0.169 5.9%
    3 24 5.66ng/ml 0.401 7.1%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human TGFBR3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213837) is designed for the quantitative measurement of Human TGFBR3 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBR3 has been pre-coated onto 96-well plates. Standards (NSO, G21-D781) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TGFBR3 amount of sample captured in plate.

  • Notes

    Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell surface chondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, non redundant role in TGF beta signaling.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human TGFBR3 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human TGFBR3 antibody 1 x 130µl
    Lyophilized recombinant Human TGFBR3 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.
  • Sequence similarities

    Contains 1 ZP domain.
  • Post-translational
    modifications

    Extensively modified by glycosaminoglycan groups (GAG).
  • Cellular localization

    Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.
  • Information by UniProt
  • Alternative names

    • Beta glycan
    • Betaglycan
    • Betaglycan proteoglycan
    • BGCAN
    • TGBR3_HUMAN
    • TGF beta receptor type 3
    • TGF beta receptor type III
    • TGF-beta receptor type 3
    • TGF-beta receptor type III
    • TGFB R3
    • TGFBR 3
    • TGFBR3
    • TGFR 3
    • TGFR-3
    • TGFR3
    • Transforming growth factor beta receptor III
    • Transforming growth factor beta receptor III betaglycan 300kDa
    • Transforming growth factor beta receptor type 3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human TGFBR3 ELISA Kit (ab213837) Standard Curve.

Protocols

References

ab213837 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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