Human TGFBR3 ELISA Kit (ab213837)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TGFBR3 ELISA Kit
See all TGF beta Receptor III/TGFBR3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 186pg/ml 8.74 = 4.7% 2 16 1370pg/ml 102.75 = 7.5% 3 16 5554pg/ml 294.36 = 5.3% Inter-assay Sample n Mean SD CV% 1 24 182pg/ml 11.83 = 6.5% 2 24 1499pg/ml 133.41 = 8.9% 3 24 5882pg/ml 376.44 = 6.4% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human TGFBR3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213837) is designed for the quantitative measurement of Human TGFBR3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBR3 has been pre-coated onto 96-well plates. Standards (NSO, G21-D781) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TGFBR3 amount of sample captured in plate.
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Notes
Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell surface chondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, non redundant role in TGF beta signaling.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human TGFBR3 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human TGFBR3 antibody 1 x 100µl Lyophilized recombinant Human TGFBR3 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors. -
Sequence similarities
Contains 1 ZP domain. -
Post-translational
modificationsExtensively modified by glycosaminoglycan groups (GAG). -
Cellular localization
Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix. - Information by UniProt
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Alternative names
- Beta glycan
- Betaglycan
- Betaglycan proteoglycan
see all -
Database links
- Entrez Gene: 7049 Human
- Omim: 600742 Human
- SwissProt: Q03167 Human
- Unigene: 482390 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213837 has not yet been referenced specifically in any publications.