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ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.
Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
|Components||1 x 4 Membranes||1 x 8 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl|
|1X Blocking Buffer||1 x 25ml||2 x 25ml|
|2000X Biotin-Conjugated Anti Cytokines||1 x 2 vials||1 x 4 vials|
|20X Wash Buffer I||1 x 10ml||1 x 20ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 16ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit|
|Angiogenesis Antibody Array Membranes||4 units||8 units|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml|
Our Abpromise guarantee covers the use of ab169809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.
ab169809 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"