Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.19 ng/ml
- Range: 0.39 ng/ml - 25 ng/ml
- Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Thioredoxin Reductase 1 ELISA Kit
Intra-assay Sample n Mean SD CV% HeLa extract 4 3.77% Inter-assay Sample n Mean SD CV% HeLa extract 4 7.3%
Sample typeSerum, Plasma, Cell culture extracts, Tissue Extracts
Assay typeSandwich (quantitative)
Range0.39 ng/ml - 25 ng/ml
Sample specific recovery Sample type Average % Range Serum 101 100% - 102% Cell culture media 98 89% - 106% Hep Plasma 101 100% - 102% EDTA Plasma 104 98% - 115% Cit plasma 99 97% - 101% Extraction Buffer 96 84% - 108%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human Thioredoxin Reductase 1 ELISA Kit (ab192150) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thioredoxin Reductase 1 protein in serum, tissue extracts, plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thioredoxin Reductase 1 with 0.14 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Thioredoxin Reductase 1 (TXNRD1) is a selenium-containing enzyme part of the thioredoxin system responsible for regulating oxidative stress and redox signaling via reduction of disulfide bonds. The dimer, TXNRD1, reduces thioredoxin and other substrates using NADPH and an FAD cofactor. TXNRD1’s involvement in protecting against oxidative stress and injury, regulation of cellular development and growth, and various other cellular processes make it an interesting target for studies of various cancers, AIDS, and other diseases. Humans express three different isozymes of thioredoxin reductase, isoform 1 is the cytosolic form, isoform 2 is the mitochondrial form, and isoform 3 is a testes specific form. This assay is specific to isoform 1 of thioredoxin reductase.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 10X Human TXNRD1 Capture Antibody 1 x 600µl 10X Human TXNRD1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human TXNRD1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Gene associated with retinoic and IFN induced mortality 12 protein
- Gene associated with retinoic and interferon induced mortality 12 protein
- GRIM 12
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
ab192150 has been referenced in 2 publications.
- Paul S et al. Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans. Funct Integr Genomics 20:191-200 (2020). PubMed: 31444657
- Cecchinato V et al. Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12/HMGB1 in Active Rheumatoid Arthritis. Front Immunol 9:2118 (2018). PubMed: 30283452