Overview

  • Product name

    Human Thioredoxin Reductase 1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 4 3.77%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 4 7.3%
  • Sample type

    Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.14 ng/ml
  • Range

    0.39 ng/ml - 24 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101 100% - 102%
    Cell culture media 98 89% - 106%
    Heparin Plasma 101 100% - 102%
    EDTA Plasma 104 98% - 115%
    Citrate Plasma 99 97% - 101%
    Extraction Buffer 96 84% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Thioredoxin Reductase 1 (TXNRD1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thioredoxin Reductase 1 protein in human serum, plasma, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Thioredoxin Reductase 1 (TXNRD1) is a selenium-containing enzyme part of the thioredoxin system responsible for regulating oxidative stress and redox signaling via reduction of disulfide bonds. The dimer, TXNRD1, reduces thioredoxin and other substrates using NADPH and an FAD cofactor. TXNRD1’s involvement in protecting against oxidative stress and injury, regulation of cellular development and growth, and various other cellular processes make it an interesting target for studies of various cancers, AIDS, and other diseases. Humans express three different isozymes of thioredoxin reductase, isoform 1 is the cytosolic form, isoform 2 is the mitochondrial form, and isoform 3 is a testes specific form. This assay is specific to isoform 1 of thioredoxin reductase.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Human TXNRD1 Capture Antibody 1 x 600µl
    10X Human TXNRD1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human TXNRD1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Alternative names

    • Gene associated with retinoic and IFN induced mortality 12 protein
    • Gene associated with retinoic and interferon induced mortality 12 protein
    • GRIM 12
    • KM 102 derived reductase like factor
    • Oxidoreductase
    • Thioredoxin reductase 1
    • Thioredoxin reductase 1 cytoplasmic
    • Thioredoxin reductase GRIM 12
    • Thioredoxin reductase TR1
    • TR
    • TR1
    • TRXR1
    • TXNR
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab192150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

This product has been referenced in:

  • Cecchinato V  et al. Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12/HMGB1 in Active Rheumatoid Arthritis. Front Immunol 9:2118 (2018). Read more (PubMed: 30283452) »
See 1 Publication for this product

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