Overview

  • Product name

    Human Thrombin-Antithrombin Complex ELISA Kit (TAT)
    See all Thrombin-Antithrombin Complex kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.9%
  • Sample type

    Cell culture supernatant, Milk, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 1 ng/ml
  • Range

    1.481 ng/ml - 120 ng/ml
  • Recovery

    95 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Thrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.


    A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Thrombin-Antithrombin Complex Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    Thrombin-Antithrombin Complex Microplate (12 x 8 well strips) 1 unit
    Thrombin-Antithrombin Complex Standard 1 vial
  • Research areas

  • Relevance

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Cellular localization

    Secreted
  • Alternative names

    • antithrombin III
    • AT3
    • AT3D
    • ATIII
    • coagulation factor II (thrombin)
    • F2
    • PRO0309
    • prothrombin
    • prothrombin B chain
    • PT
    • RPRGL2
    • serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
    • serine protease
    • serine-cysteine proteinase inhibitor clade C member 1
    • serpin C1
    • serpin peptidase inhibitor, clade C (antithrombin), member 1
    • SERPINC1
    • TAT
    • THPH1
    • THPH7
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • TAT measured in biological fluids, with background signal subtracted (duplicates +/- SD).

  • Representative Standard Curve Using ab108907.

Protocols

References

This product has been referenced in:

  • Park H  et al. Polymeric nano-shielded islets with heparin-polyethylene glycol in a non-human primate model. Biomaterials 171:164-177 (2018). Read more (PubMed: 29698867) »
  • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). Read more (PubMed: 29599912) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Abreviews
Human Thrombin-Antithrombin Complex ELISA Kit Abcam Review:

An experienced laboratory technician completed this Thrombin-Antithrombin ELISA from Abcam, catalog number AB108907, Lot GR256365-9. The goal of the experiment was to analyze the linearity of dilutions of 3 different rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross-reactivity for rhesus macaque.

Overall, the kit, like other Abcam ELISA kits, was easy to follow, and was straight-forward in directions, while also being brief. The laboratory technician had no problem understanding any parts of the protocol.

Our experiment consisted of using EDTA-prepared rhesus macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted 1:5, 1:10, 1:20, and 1:40 in the kit’s diluent, and tested in duplicate, as were the standards. The rest of the assay was performed to protocol’s specifications.

The kit recommended 5 standard dilutions. We noted that the highest concentration all but reaches 1.0 OD when assay recommendations are followed. We recommend using a higher-sensitivity TMB substrate or extending TMB incubation time considerably.

The tested sample dilutions all had blank-subtracted OD less than 0.05. Spiked human protein was recoverable at 1:40 (60%).

Overall, we do not believe this kit is capable of detecting TAT complexes in plasma of rhesus macaque.

Abcam user community

Verified customer

Submitted Jun 06 2016

Abreviews
Stored serum samples (-20) were used from rhesus macaque monkeys (NHP), 1:5 sample dilution with Abcam TAT ELISA kit used as per protocol to measure Thrombin-Anti-Thrombin complexes post-transplant, see attached image.
Colour development was faster (3-5m) than the c.20 mins anticipated in the protocol.

Abcam user community

Verified customer

Submitted Nov 02 2015

Question
Answer



All our kits are guaranteed for the first six months. But having said that, stored at the right conditions, the reagents could last up to a year.

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Question
Answer





TAT Complexes Standard: Reconstitute the 240 ng of human TAT Complexes Standard with 2 ml of MIX Diluent to generate a standard solution of 120 ng/ml. Allow the standard to sit for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting the Standard solution 1:3 with MIX Diluent to produce 40, 13.33, 4.44, and 1.48 ng/ml. MIX Diluent serves as the zero standard




(0 ng/ml). Any remaining solution should be frozen at -200C. Standard Point
Dilution
[TAT] (ng/ml)

P1
1 part Standard Solution (120 ng/ml)
120.00

P2
1 part P1 + 2 parts MIX Diluent
40.00

P3
1 part P2 + 2 parts MIX Diluent
13.33

P4
1 part P3 + 2 parts MIX Diluent
4.44

P5
1 part P4 + 2 parts MIX Diluent
1.48

P6
MIX Dilutent
0.00

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Question
Answer

Thank you for contacting us.

The catalogue number is ab108907

https://www.abcam.com/Thrombin-Antithrombin-Complex-TAT-Human-ELISA-Kit-ab108907.html

Please find attached the datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

I am sorry you could not find this information in the protocol book. The range for this kit is stated on our datasheet and corresponds to 0.48 ng/ml - 120 ng/ml.

I hope this helps and if we can assist further, please do not hesitate to contact us.

Read More

Answer


The range for the strandard is from 1.48 to 120 ng/ml, as shown on pages 8 and 9 of the booklet: https://www.abcam.com/ps/products/108/ab108907/documents/ab108907%20Thrombin%20Antithrombin%20Complex%20Human%20ELISA%20Kit%20(Web)%20v2.pdf.

I hope this information is the answer toyour question. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting Abcam.
Unfortunately ab108907 will not work well with mouse samples. We do have a few other kits that work on mouse that may be suitable though:
https://www.abcam.com/Antithrombin-III-Mouse-ELISA-Kit-ab108800.html
https://www.abcam.com/Antithrombin-III-Human-ELISA-Kit-ab108801.html

If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

I am pleased to hear the kit has worked well for you. In answer to your questions:

1. Yes, as stated in the protocol the standard and biotinylated antibody can be kept at -20oC once reconstituted. Although I would recommend to avoid freeze thaw cycles as much as possible (eg by aliquotting).

2. I can confirm we have stock at the moment.

I hope this will be helpful to you. Good luck with your experiments and please do not hesitate to contact us if you require anything further.

Read More

Question
Answer

Thank you for your patience.

I have heard back from the lab with the following information:

We have not yet tested the kit in whole blood. However, we tested bovine samples. The cross-reactivity is very low, around 2%.

Thus, the kit might not work well with bovine samples - whether whole blood or plasma/serum.

I am sorry to not have better news for you.

But in general, I wanted to point out to you that for UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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1-10 of 16 Abreviews or Q&A

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