Human Thrombin-Antithrombin Complex ELISA Kit Abcam Review:
An experienced laboratory technician completed this Thrombin-Antithrombin ELISA from Abcam, catalog number AB108907, Lot GR256365-9. The goal of the experiment was to analyze the linearity of dilutions of 3 different rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross-reactivity for rhesus macaque.
Overall, the kit, like other Abcam ELISA kits, was easy to follow, and was straight-forward in directions, while also being brief. The laboratory technician had no problem understanding any parts of the protocol.
Our experiment consisted of using EDTA-prepared rhesus macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted 1:5, 1:10, 1:20, and 1:40 in the kit’s diluent, and tested in duplicate, as were the standards. The rest of the assay was performed to protocol’s specifications.
The kit recommended 5 standard dilutions. We noted that the highest concentration all but reaches 1.0 OD when assay recommendations are followed. We recommend using a higher-sensitivity TMB substrate or extending TMB incubation time considerably.
The tested sample dilutions all had blank-subtracted OD less than 0.05. Spiked human protein was recoverable at 1:40 (60%).
Overall, we do not believe this kit is capable of detecting TAT complexes in plasma of rhesus macaque.
An experienced laboratory technician completed this Thrombin-Antithrombin ELISA from Abcam, catalog number AB108907, Lot GR256365-9. The goal of the experiment was to analyze the linearity of dilutions of 3 different rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross-reactivity for rhesus macaque.
Overall, the kit, like other Abcam ELISA kits, was easy to follow, and was straight-forward in directions, while also being brief. The laboratory technician had no problem understanding any parts of the protocol.
Our experiment consisted of using EDTA-prepared rhesus macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted 1:5, 1:10, 1:20, and 1:40 in the kit’s diluent, and tested in duplicate, as were the standards. The rest of the assay was performed to protocol’s specifications.
The kit recommended 5 standard dilutions. We noted that the highest concentration all but reaches 1.0 OD when assay recommendations are followed. We recommend using a higher-sensitivity TMB substrate or extending TMB incubation time considerably.
The tested sample dilutions all had blank-subtracted OD less than 0.05. Spiked human protein was recoverable at 1:40 (60%).
Overall, we do not believe this kit is capable of detecting TAT complexes in plasma of rhesus macaque.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jun 06 2016