Human Thrombomodulin ELISA Set (without plates) (ab47358)

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Overview

  • Product name

    Human Thrombomodulin ELISA Set (without plates)

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 0.38 ng/ml

  • Range

    0.625 ng/ml - 20 ng/ml

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Thrombomodulin Human in vitro ELISA Set is designed for the quantitative measurement of Thrombomodulin in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human Thrombomodulin in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    These reagents are intended for research use only, not for diagnostic purposes.

     

    The Thrombomodulin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human Thrombomodulin.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Detection biotinylated anti-Thrombomodulin antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    Streptavidin-HRP 1 vial 2 vials 3 vials
    Thrombomodulin Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    Thrombomodulin standard 5 vials 10 vials 15 vials

  • Research areas

  • Relevance

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. Endothelial cells are unique in synthesizing thrombomodulin. The thrombomodulin/thrombin complex is responsible for the conversion of protein C to the activated protein C (protein Ca), which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Defects in Thrombomodulin may be a cause of thromboembolic disease.

  • Cellular localization

    single-pass type I membrane protein

  • Alternative names

    • CD141
    • CD141 antigen
    • Fetomodulin
    • THBD
    • THRM
    • TM
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab47358 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Thrombomodulin Human ELISA Set (ab47358)
      Thrombomodulin Human ELISA Set (ab47358)
      Thrombomodulin Standard curve ranging from 0.625 to 20 ng/ml

    References

    ab47358 has not yet been referenced specifically in any publications.

    Publishing research using ab47358? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    what kind of protein is ab47358 standard? from human or recombinent protein? If it is recombinent protein, would you please help us to check the sequence? (fragment or full length?)

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    Answer

    Thank you for contacting us. The standard protein in the kit ab47358 is a recombinant human thrombomodulin, carboxyl-terminal truncated form - lacks the putative transmembrane and the cytoplasmic domains (~38 amino acids) - purified from a Chinese Hamster Ovary (CHO) cell line, using reverse phase, ion exchange chromatography and sizing columns. By SDS-PAGE it migrates at approximately 68 kDa. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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