Human Thrombomodulin ELISA Set (without plates) (ab47358)

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Thrombomodulin Human ELISA Set (ab47358)

    Overview

    • Product name

      Human Thrombomodulin ELISA Set (without plates)

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 0.38 ng/ml

    • Range

      0.625 ng/ml - 20 ng/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s Thrombomodulin Human in vitro ELISA Set is designed for the quantitative measurement of Thrombomodulin in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

       

      This ELISA Set is intended to measure human Thrombomodulin in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

      These reagents are intended for research use only, not for diagnostic purposes.

       

      The Thrombomodulin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

       

      Recognizes both natural and recombinant human Thrombomodulin.

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 5 x 96 tests 10 x 96 tests 15 x 96 tests
      Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
      Detection biotinylated anti-Thrombomodulin antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
      Streptavidin-HRP 1 vial 2 vials 3 vials
      Thrombomodulin Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
      Thrombomodulin standard 5 vials 10 vials 15 vials

    • Research areas

    • Relevance

      Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. Endothelial cells are unique in synthesizing thrombomodulin. The thrombomodulin/thrombin complex is responsible for the conversion of protein C to the activated protein C (protein Ca), which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Defects in Thrombomodulin may be a cause of thromboembolic disease.

    • Cellular localization

      single-pass type I membrane protein

    • Alternative names

      • CD141
      • CD141 antigen
      • Fetomodulin
      • THBD
      • THRM
      • TM
      see all

    • Database links

      Associated products

        Applications

        The Abpromise guarantee

        Our Abpromise guarantee covers the use of ab47358 in the following tested applications.

        The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

        Application Abreviews Notes
        Sandwich ELISA
        Use at an assay dependent concentration.
        Notes
        Sandwich ELISA
        Use at an assay dependent concentration.

        Images

        • Thrombomodulin Human ELISA Set (ab47358)
          Thrombomodulin Human ELISA Set (ab47358)
          Thrombomodulin Standard curve ranging from 0.625 to 20 ng/ml

        References (0)

        Publishing research using ab47358? Please let us know so that we can cite the reference in this datasheet.

        ab47358 has not yet been referenced specifically in any publications.

        Customer reviews and Q&As

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        Question

        what kind of protein is ab47358 standard? from human or recombinent protein? If it is recombinent protein, would you please help us to check the sequence? (fragment or full length?)

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        Answer

        Thank you for contacting us. The standard protein in the kit ab47358 is a recombinant human thrombomodulin, carboxyl-terminal truncated form - lacks the putative transmembrane and the cytoplasmic domains (~38 amino acids) - purified from a Chinese Hamster Ovary (CHO) cell line, using reverse phase, ion exchange chromatography and sizing columns. By SDS-PAGE it migrates at approximately 68 kDa. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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