Overview

  • Product name

    Human Thrombopoietin ELISA Kit
    See all Thrombopoietin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 3 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Seum 5 5%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 83 81% - 84%
    Cell culture media 89 84% - 84%
    Hep Plasma 98 97% - 100%
    EDTA Plasma 101 96% - 109%
    Cit plasma 84 80% - 90%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Thrombopoietin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombopoietin protein in human serum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS - 14.7 pg/mL
    Samples in Sample Diluent 25BP - 11.0 pg/mL
    Samples in Sample Diluent 10BP - 11.2 pg/mL

  • Notes

    Thrombopoietin is a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. Thrombopoietin acts at a late stage of megakaryocyte development.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Thrombopoietin Capture Antibody 1 x 600µl
    10X Human Thrombopoietin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human Thrombopoietin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS 1 x 100ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • Involvement in disease

    Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
  • Sequence similarities

    Belongs to the EPO/TPO family.
  • Domain

    Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C mpl ligand
    • C-mpl ligand
    • Megakaryocyte colony stimulating factor
    • Megakaryocyte colony-stimulating factor
    • Megakaryocyte growth and development factor
    • Megakaryocyte stimulating factor
    • MGC163194
    • MGDF
    • MKCSF
    • ML
    • MPL ligand
    • MPLLG
    • Myeloproliferative leukemia virus oncogene ligand
    • Prepro thrombopoietin
    • THCYT1
    • THPO
    • Thrombopoietin
    • Thrombopoietin nirs variant 1
    • TPO
    • TPO_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab219632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, cell culture media neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 47.1 pg/mL in neat serum, 79.6 pg/mL in neat plasma (citrate), 109 pg/mL in plasma (heparin), and 54.1 pg/mL in plasma (EDTA). Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 60.1 pg/mL with a range of 19.6 – 151 pg/mL. Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.

Protocols

References

This product has been referenced in:

  • Ishii M  et al. Reduction in thrombogenic activity and thrombocytopenia after transcatheter aortic valve implantation - The ATTRACTIVE-TTAS study. Int J Cardiol Heart Vasc 23:100346 (2019). Read more (PubMed: 30976651) »
See 1 Publication for this product

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