Human Thrombospondin 1 ELISA Kit (ab193716)
Overview
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Product name
Human Thrombospondin 1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6 ng/ml -
Range
6 ng/ml - 2000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91.67 79% - 113% Plasma 83.27 67% - 109% Cell culture media 86.97 73% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Thrombospondin 1 Human ELISA Kit (ab193716) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombospondin 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Thrombospondin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Thrombospondin 1 present in the samples. The wells are washed and biotinylated Human Thrombospondin 1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Thrombospondin 1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Biotinylated Human Thrombospondin detection antibody (lyophilized) 2 vials Human Thrombospondin Standards (lyophilized) 2 vials Pre-coated Human Thrombospondin Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. -
Cellular localization
Endoplasmic reticulum. Sarcoplasmic reticulum. - Information by UniProt
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Alternative names
- THBS
- THBS 1
- Thbs1
see all -
Database links
- Entrez Gene: 7057 Human
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
Images
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Typical Standard CurveTypical Standard Curve using ab193716 Thrombospondin 1 Human ELISA Kit.
References
ab193716 has not yet been referenced specifically in any publications.
