Human Thrombospondin 1 ELISA Kit (ab193716)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 6 ng/ml
    • Range: 6 ng/ml - 2000 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Thrombospondin 1 ELISA Kit
      See all Thrombospondin kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      6 ng/ml

    • Range

      6 ng/ml - 2000 ng/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 91.67 79% - 113%
      Plasma 83.27 67% - 109%
      Cell culture media 86.97 73% - 108%

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Thrombospondin 1 Human ELISA Kit (ab193716) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombospondin 1 in serum, plasma and cell culture supernatants.


      This assay employs an antibody specific for Human Thrombospondin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Thrombospondin 1 present in the samples. The wells are washed and biotinylated Human Thrombospondin 1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Thrombospondin 1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Typical Standard Curve using ab193716 Thrombospondin 1 Human ELISA Kit.

    References (0)

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