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    human-thrombospondin-1-elisa-kit-ab193716.pdf

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Human Thrombospondin 1 ELISA Kit (ab193716)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 6 ng/ml
    • Range: 6 ng/ml - 2000 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human Thrombospondin 1 ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      6 ng/ml
    • Range

      6 ng/ml - 2000 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 91.67 79% - 113%
      Plasma 83.27 67% - 109%
      Cell culture media 86.97 73% - 108%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Thrombospondin 1 Human ELISA Kit (ab193716) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombospondin 1 in serum, plasma and cell culture supernatants.


      This assay employs an antibody specific for Human Thrombospondin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Thrombospondin 1 present in the samples. The wells are washed and biotinylated Human Thrombospondin 1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Thrombospondin 1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      5X Assay Diluent D 1 x 15ml
      700X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent B 1 x 15ml
      Biotinylated Human Thrombospondin detection antibody 2 vials
      Human Thrombospondin Standards (lyophilized) 2 vials
      Pre-coated Human Thrombospondin Microplate (12 strips x 8 wells) 1 unit
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Astrocyte marker
      • Cardiovascular
      • Blood
      • Fibrinolysis / Thrombolysis
      • Cardiovascular
      • Blood
      • Platelets
      • Cardiovascular
      • Blood
      • Coagulation
      • Regulatory
      • Neuroscience
      • Cell Adhesion Proteins
      • Cytoskeletal Proteins
      • Regulation
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Secreted
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic inhibitory factors
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
    • Function

      Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.
    • Sequence similarities

      Belongs to the thrombospondin family.
      Contains 2 EGF-like domains.
      Contains 1 laminin G-like domain.
      Contains 1 TSP C-terminal (TSPC) domain.
      Contains 3 TSP type-1 domains.
      Contains 8 TSP type-3 repeats.
      Contains 1 VWFC domain.
    • Cellular localization

      Endoplasmic reticulum. Sarcoplasmic reticulum.
    • Target information above from: UniProt accession P07996 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Thbs1
      • Thrombospondin-1
      • TSP
      • Tsp1
      • TSP1_HUMAN
      see all
    • Database links

      • Entrez Gene: 7057 Human
      • Omim: 188060 Human
      • SwissProt: P07996 Human
      • Unigene: 164226 Human
      • Unigene: 732539 Human

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Typical Standard Curve using ab193716 Thrombospondin 1 Human ELISA Kit.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab193716? Please let us know so that we can cite the reference in this datasheet.

      ab193716 has been referenced in 1 publication.

      • Herken K  et al. Age-Dependent Control of Collagen-Dependent Platelet Responses by Thrombospondin-1-Comparative Analysis of Platelets from Neonates, Children, Adolescents, and Adults. Int J Mol Sci 22:N/A (2021). PubMed: 34063076

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