Product nameHuman Thrombospondin 1 ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range6 ng/ml - 2000 ng/ml
Sample specific recovery Sample type Average % Range Serum 91.67 79% - 113% Plasma 83.27 67% - 109% Cell culture media 86.97 73% - 108%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Thrombospondin 1 Human ELISA Kit (ab193716) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombospondin 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Thrombospondin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Thrombospondin 1 present in the samples. The wells are washed and biotinylated Human Thrombospondin 1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Thrombospondin 1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Biotinylated Human Thrombospondin detection antibody (lyophilized) 2 vials Human Thrombospondin Standards (lyophilized) 2 vials Pre-coated Human Thrombospondin Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.
Sequence similaritiesBelongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.
Cellular localizationEndoplasmic reticulum. Sarcoplasmic reticulum.
- Information by UniProt
- THBS 1
Our Abpromise guarantee covers the use of ab193716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193716 has not yet been referenced specifically in any publications.