Overview

  • Product name

    Human Thymidine Kinase 1 ELISA Kit
    See all Thymidine Kinase 1/TK1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 5 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 7.8%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    6.1 pg/ml
  • Range

    23.5 pg/ml - 3500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 95% - 104%
    Serum 85 75% - 91%
    Cell culture extracts 102 86% - 120%
    EDTA Plasma 80 80% - 81%
    Citrate Plasma 80 75% - 82%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Thymidine Kinase 1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thymidine kinase 1 protein in human plasma, serum, and cell culture lysate and supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Thymidine kinase 1, or TK1, is a 234- amino acid, zinc binding protein encoded by the Thymidine Kinase 1 gene. The primary function of Thymidine Kinase 1 is the phosphorylation of thymidine to form deoxythymidine 5’-phosphate, dTMP, and is necessary for DNA replication. The activity of Thymidine Kinase 1 is highest during the S phase of cells, and lowers during the G2 phase. Two forms of Thymidine Kinase 1 have been identified, one in the cytosol and the other in mitochondria, which is known as TK2. The presence of Thymidine Kinase 1 in serum may be a biomarker for certain types of cancer.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab223595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Thymidine Kinase 1 in human PC-3, MCF7, and SH-SY5Y cell extracts based on a 16 μg/mL extract load. The concentrations of Thymidine Kinase 1 were measured in duplicate and interpolated from the Thymidine Kinase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thymidine Kinase 1 concentration was determined to be 1251 pg/ml in PC-3, 585 pg/mL in MCF7, 721 pg/mL in SH-SY5Y.

  • The concentrations of Thymidine Kinase 1 were measured in duplicates, interpolated from the Thymidine Kinase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 25% and HUVEC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab223595 has not yet been referenced specifically in any publications.

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