Human Thyroglobulin ELISA Kit (ab155441)

Overview

  • Product name
    Human Thyroglobulin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.22 ng/ml
  • Range
    0.205 ng/ml - 50 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 115.5 105% - 125%
    Plasma 98.71 88% - 106%
    Cell culture media 96.01 88% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thyroglobulin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Thyroglobulin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Thyroglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thyroglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thyroglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thyroglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human Thyroglobulin 2 vials
    Recombinant Human Thyroglobulin Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    Thyroglobulin Microplate (12 x 8 wells) 1 unit
    TMB One-Step Substrate Reagent 1 x 12ml
  • Relevance
    Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).
  • Cellular localization
    Secreted.
  • Alternative names
    • TG
  • Database links

Applications

Our Abpromise guarantee covers the use of ab155441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155441 -  Assay Diluent C.

Protocols

References

ab155441 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Thyroglobuline ELISA kit tested with cat samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The assay performed well in terms of generating a standard curve and ease of use. We ran the assay twice, half a plate at a time. It is difficult to be certain based on what we have done thus far whether the assay worked for feline samples. Certainly a few of the samples were positive but these weren't from hyperthyroid cats but from young adult normal individuals. Whether this is a spurious result in a couple of wells (and otherwise a lack of antibody cross-reactivity) or represents a real finding is unclear at present. I suspect that some of the samples are positive and we now plan to use the assay again to try to characterise better why (i.e. in which patients) this is the case.

Dr. Frederique Guesdon

Verified customer

Submitted Mar 01 2016

Answer

Thank you for contacting us.

Please note that although this kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay Diluent B to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by you empirically. In brief, a lysis buffer must meet the following specifications: A) has relatively low salt content (700 mM or less) B) does not contain sodium azide C) does not contain >0.1% SDS D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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