Human Thyroglobulin ELISA Kit (ab155441)
Key features and details
- Sensitivity: 0.22 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thyroglobulin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.22 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 115.5 105% - 125% Plasma 98.71 88% - 106% Cell culture media 96.01 88% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Thyroglobulin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Thyroglobulin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Thyroglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thyroglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thyroglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thyroglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human Thyroglobulin 2 vials Recombinant Human Thyroglobulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml Thyroglobulin Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Relevance
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
Cellular localization
Secreted. -
Alternative names
- TG
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Database links
- Entrez Gene: 7038 Human
- Omim: 188450 Human
- SwissProt: P01266 Human
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155441 has been referenced in 2 publications.
- Threapleton DE et al. Prenatal and Postpartum Maternal Iodide Intake from Diet and Supplements, Urinary Iodine and Thyroid Hormone Concentrations in a Region of the United Kingdom with Mild-to-Moderate Iodine Deficiency. Nutrients 13:N/A (2021). PubMed: 33466826
- Tani N et al. Thyroid-related hormones as potential markers of hypoxia/ischemia. Hum Cell 33:545-558 (2020). PubMed: 32146707