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  1. Link

    human-thyroglobulin-elisa-kit-ab178631.pdf

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Human Anti-Thyroglobulin ELISA Kit (ab178631)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (1)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.5 U/ml
    • Range: 2 U/ml - 128 U/ml
    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

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    Overview

    • Product name

      Human Anti-Thyroglobulin ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Pos. Serum 20 2.1%
      Pos. Serum 20 1.2%
      Inter-assay
      Sample n Mean SD CV%
      Pos. Serum 12 59.7U/ml 5.7%
      Pos. Serum 12 28.9U/ml 8.5%
      Neg. Serum 12 7.3U/ml 3.9%
    • Sample type

      Serum, Hep Plasma, Cit plasma
    • Assay type

      Indirect
    • Sensitivity

      < 0.5 U/ml
    • Range

      2 U/ml - 128 U/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s anti-Thyroglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroglobulin in Human serum or plasma.


      A 96-well plate has been precoated with Human thyroglobulin to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroglobulin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroglobulin sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      20X Washing Solution White cap 1 x 50ml
      Cover Foil 1 unit
      IgG Sample Diluent 1 x 100ml
      Stop Solution red cap 1 x 15ml
      Strip holder 1 unit
      Thyroglobulin Anti-IgG HRP Conjugate 1 x 20ml
      Thyroglobulin Coated Microplate (12 x 8 wells) 1 unit
      Thyroglobulin Negative Control 1 x 2ml
      Thyroglobulin Positive Control 1 x 2ml
      Thyroglobulin Standard A - 0 U/mL 1 x 2ml
      Thyroglobulin Standard B - 2 U/mL 1 x 2ml
      Thyroglobulin Standard C - 4 U/mL 1 x 2ml
      Thyroglobulin Standard D - 8 U/mL 1 x 2ml
      Thyroglobulin Standard E - 32 U/mL 1 x 2ml
      Thyroglobulin Standard F - 128 U/mL 1 x 2ml
      TMB Substrate Solution Yellow cap 1 x 15ml

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab178631

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab178631? Please let us know so that we can cite the reference in this datasheet.

      ab178631 has been referenced in 1 publication.

      • Landriscina M  et al. RAS/BRAF mutational status in familial non-medullary thyroid carcinomas: A retrospective study. Oncol Lett 10:1875-1881 (2015). PubMed: 26622768

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