Human Anti-Thyroglobulin ELISA Kit (ab178631)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human Anti-Thyroglobulin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pos. Serum 20 2.1% Pos. Serum 20 1.2% Inter-assay Sample n Mean SD CV% Pos. Serum 12 59.7U/ml 5.7% Pos. Serum 12 28.9U/ml 8.5% Neg. Serum 12 7.3U/ml 3.9% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
< 0.5 U/ml -
Range
2 U/ml - 128 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Thyroglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroglobulin in Human serum or plasma.
A 96-well plate has been precoated with Human thyroglobulin to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroglobulin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroglobulin sample captured in plate.
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Tested applications
Suitable for: Indirect ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit Thyroglobulin Anti-IgG HRP Conjugate 1 x 20ml Thyroglobulin Coated Microplate (12 x 8 wells) 1 unit Thyroglobulin Negative Control 1 x 2ml Thyroglobulin Positive Control 1 x 2ml Thyroglobulin Standard A - 0 U/mL 1 x 2ml Thyroglobulin Standard B - 2 U/mL 1 x 2ml Thyroglobulin Standard C - 4 U/mL 1 x 2ml Thyroglobulin Standard D - 8 U/mL 1 x 2ml Thyroglobulin Standard E - 32 U/mL 1 x 2ml Thyroglobulin Standard F - 128 U/mL 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml
Applications
Our Abpromise guarantee covers the use of ab178631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab178631
Protocols
References
This product has been referenced in:
- Landriscina M et al. RAS/BRAF mutational status in familial non-medullary thyroid carcinomas: A retrospective study. Oncol Lett 10:1875-1881 (2015). Read more (PubMed: 26622768) »
