Overview

  • Product name

    Human Thyroid Stimulating Hormone ELISA Kit (TSH)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 4 pg/ml
  • Range

    2.744 pg/ml - 2000 pg/ml
  • Recovery

    86 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82.76 70% - 90%
    Serum 97.89 75% - 120%
    Plasma 78.59 67% - 86%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TSH Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TSH in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TSH 2 vials
    Recombinant Human TSH Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TSH Microplate (12 x 8 wells) 1 unit
  • Research areas

  • Relevance

    Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
  • Cellular localization

    Secreted
  • Alternative names

    • Anterior pituitary glycoprotein hormones common subunit alpha
    • CG ALPHA
    • CGA
    • CGA protein
    • Choriogonadotropin alpha chain
    • Chorionic gonadotrophin alpha subunit
    • Chorionic gonadotropin alpha polypeptide
    • Follicle stimulating hormone alpha chain
    • Follitropin alpha chain
    • FSH alpha
    • FSHA
    • Glycoprotein hormones alpha chain
    • Glycoprotein hormones alpha polypeptide
    • GPHa
    • GPHA1
    • HCG
    • LHA
    • LSH alpha
    • Luteinizing hormone alpha chain
    • Lutropin alpha chain
    • Thyroid stimulating hormone
    • Thyroid stimulating hormone alpha chain
    • Thyroid stimulating hormone beta
    • Thyroid stimulating hormone beta subunit
    • Thyroid stimulating hormone subunit beta
    • Thyroid Stimulation Hormone
    • Thyrotropin
    • Thyrotropin alfa
    • Thyrotropin alpha chain
    • Thyrotropin beta subunit
    • Thyrotropin subunit beta
    • TSH A
    • TSH alpha
    • TSH B
    • TSH beta
    • TSHA
    • TSHB
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100660

  • Representative standard curve using ab100660

Protocols

References

ab100660 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us at Abcam Scientific Support,

I am happy to help explain the vial contents for the ELH-TSH standard. Each vial is enough to run a duplicate standard curve. Given the concentration and volumes described in the manual each vial contains 20ng of standard.

Please let me know if I can offer any additional assistance. Take care.

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Question
Answer


I am very pleased to hear you would like to accept our offer and test ab100660 in monkey. This code will give you $XXX off your next order before the expiration date. To redeem this offer, please submit an Abreview for monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial

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Answer

Thank you for your response.

These products are all guaranteed to work for a period of six months from date of delivery if stored at +4C. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

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Question
Answer

Thank you for contacting us.

The catalog numbers ab108659 and ab108660 come from the same product line and are easy to compare. The TSH ELISA Kit ab108659 comes with standards in the range of 0-25 uIU/ml, while the TSH ELISA ab108660 is considered ultrasensitive at comes with standards in the range of 0-10 uIU/ml. The run time for the ultrasensitive kit is 120 & 20 minutes while the standard kit is 60 & 20 minutes.

The kit ab108682 has incubation times of 60 minutes and 15 minutes with the first incubation to be conducted at 37º C. The standards for this test come in the range of 0-20 uIU/ml.

The kit ab100660 measures TSH in picograms and has a range of 2.744 pg/ml - 2000 pg/ml. Incubation times for this kit are 2.5 hours at room temperature to overnight at +4C. The standards for this test come in the range of 0-2000 pg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

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