Overview

  • Product name

    Human Thyroid Stimulating Hormone ELISA Kit (TSH)
  • Detection method

    Colorimetric
  • Sample type

    Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.2 U/ml
  • Range

    0.2 µlU/ml - 25 µlU/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Thyroid Stimulating Hormone in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroid Stimulating Hormone in Human serum.

    A 96-well plate has been precoated with anti-Thyroid Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Thyroid Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Thyroid Stimulating Hormone HRP conjugate binds to the antibody-Thyroid Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Thyroid Stimulating Hormone in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Thyroid Stimulating Hormone Coated Microplate (12 x 8 wells) 1 x 96 units
    Anti-Thyroid Stimulating Hormone HRP Conjugate Reagent 1 x 13ml
    Stop Solution 1 x 11ml
    TMB Reagent 1 x 11ml
    TSH Standard 0 - 0 µIU/mL (Lyophilized) 1 vial
    TSH Standard 5 - 10 µIU/mL (Lyophilized) 1 vial
    TSH Standard 1 - 0.1 µIU/mL (Lyophilized) 1 vial
    TSH Standard 2 - 0.5 µIU/mL (Lyophilized) 1 vial
    TSH Standard 3 - 2.0 µIU/mL (Lyophilized) 1 vial
    TSH Standard 4 - 5 µIU/mL (Lyophilized) 1 vial
  • Research areas

  • Alternative names

    • CG alpha
    • CGA protein
    • Choriogonadotropin alpha chain
    • Chorionic Gonadotrophin Alpha Subunit
    • Chorionic gonadotropin alpha polypeptide
    • Follicle stimulating hormone alpha chain
    • Follitropin alpha chain
    • FSH Alpha
    • FSHA
    • Glycoprotein hormones alpha chain
    • Glycoprotein hormones alpha polypeptide
    • GPHa
    • GPHA1
    • HCG
    • LHA
    • LSH alpha
    • Luteinizing hormone alpha chain
    • Lutropin alpha chain
    • Thyroid stimulating hormone alpha chain
    • Thyroid Stimulating Hormone beta
    • Thyroid stimulating hormone beta subunit
    • Thyroid stimulating hormone subunit beta
    • Thyroid Stimulation Hormone
    • Thyrotropin
    • Thyrotropin alfa
    • Thyrotropin alpha chain
    • Thyrotropin beta subunit
    • Thyrotropin subunit beta
    • TSH A
    • TSH Alpha
    • TSH B
    • TSH beta
    • TSHA
    • TSHB
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative standard curve using ab108660

Protocols

References

This product has been referenced in:

  • Kotb El-Sayed MI  et al. Neural and Endocrinal Pathobiochemistry of Vitiligo: Comparative Study for a Hypothesized Mechanism. Front Endocrinol (Lausanne) 9:197 (2018). Read more (PubMed: 29922226) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your response.

These products are all guaranteed to work for a period of six months from date of delivery if stored at +4C. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



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Question
Answer

Thank you for contacting us.

The catalog numbers ab108659 and ab108660 come from the same product line and are easy to compare. The TSH ELISA Kit ab108659 comes with standards in the range of 0-25 uIU/ml, while the TSH ELISA ab108660 is considered ultrasensitive at comes with standards in the range of 0-10 uIU/ml. The run time for the ultrasensitive kit is 120 & 20 minutes while the standard kit is 60 & 20 minutes.

The kit ab108682 has incubation times of 60 minutes and 15 minutes with the first incubation to be conducted at 37º C. The standards for this test come in the range of 0-20 uIU/ml.

The kit ab100660 measures TSH in picograms and has a range of 2.744 pg/ml - 2000 pg/ml. Incubation times for this kit are 2.5 hours at room temperature to overnight at +4C. The standards for this test come in the range of 0-2000 pg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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