Key features and details
- Sensitivity: 0.5 pg/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameHuman Thyroxine ELISA Kit (fT4)
See all Thyroxine (T4) kits
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Thyroxine (fT4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of free Thyroxine in Human serum and plasma.
A 96-well plate has been precoated with anti-Thyroxine antibodies. Samples and the Thyroxine-HRP conjugate are added to the wells, where Thyroxine in the sample competes with the added
Thyroxine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine in the sample and the intensity is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 1X Thyroxine-HRP Conjugate 1 x 12ml 50X Wash Solution 1 x 20ml Anti-Thyroxine IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Thyroxine Standard 0 – 0.0 pg/mL 1 x 1ml Thyroxine Standard 1 – 3.0 pg/mL 1 x 1ml Thyroxine Standard 2 – 9.5 pg/mL 1 x 1ml Thyroxine Standard 3 – 21.0 pg/mL 1 x 1ml Thyroxine Standard 4 – 36.0 pg/mL 1 x 1ml Thyroxine Standard 5 – 70.0 pg/mL 1 x 1ml TMB Substrate Solution 1 x 15ml
- SwissProt: P05543 Human
ab108686 has been referenced in 2 publications.
- Zheng R et al. A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher-Neuhäuser syndrome. Mol Med Rep 18:261-267 (2018). PubMed: 29749493
- Kotb El-Sayed MI et al. Neural and Endocrinal Pathobiochemistry of Vitiligo: Comparative Study for a Hypothesized Mechanism. Front Endocrinol (Lausanne) 9:197 (2018). PubMed: 29922226