Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Tick-borne encephalitis virus IgG ELISA Kit (TBE)
See all Tick-Borne Encephalitis (TBE/FSME) Virus IgG kits
Intra-assay Sample n Mean SD CV% Standard B 7 6.8% Standard C 8 5.7% Standard D 8 4.7% Standard E 8 4.2% Inter-assay Sample n Mean SD CV% Standard B 14 7.35% Standard C 16 8.3% Standard D 15 7.8% Standard E 16 6.7%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Tick-borne encephalitis (TBE) virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Tick-borne encephalitis virus in Human serum and plasma.
A 96-well plate has been precoated with Tick-borne encephalitis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Tick-borne encephalitis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tick-borne encephalitis IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TBE virus (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit TBE virus anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml TBE virus IgG Standard A – 0 U/mL Yellow Blue cap 1 x 2ml TBE virus IgG Standard B – 50 U/mL Yellow, Green Cap 1 x 2ml TBE virus IgG Standard C – 130 U/mL Yellow, Yellow cap 1 x 2ml TBE virus IgG Standard D – 200 U/mL Yellow, Red cap 1 x 2ml TBE virus IgG Standard E – 300 U/mL Yellow, White cap 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml
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