• Product name
    Human TIE2 ELISA Kit
    See all TIE2 kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Serum 6 7.1%
    Sample n Mean SD CV%
    Serum 6 6.3%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.03 ng/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Recovery

    87 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TIE2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TIE2 concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    TIE2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an biotinylated TIE2 detection antibody and the microplate is then incubated overnight at 4 ºC. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of TIE2 captured on the plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human TIE-2 polyclonal antibody 1 x 70µl
    Human TIE2 Standard lyophilized 2 vials
    Polyclonal anti-Human TIE2 coated microplate (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
  • Tissue specificity
    Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • Involvement in disease
    Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
    Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • Angiopoietin-1 receptor
    • CD202b
    • CD202b antigen
    • EC
    • hTIE2
    • p140 TEK
    • soluble TIE2 variant 1
    • soluble TIE2 variant 2
    • Tek
    • TEK tyrosine kinase, endothelial
    • TIE 2
    • TIE2_HUMAN
    • Tunica interna endothelial cell kinase
    • Tyrosine-protein kinase receptor TEK
    • Tyrosine-protein kinase receptor TIE 2
    • Tyrosine-protein kinase receptor TIE-2
    • venous malformations, multiple cutaneous and mucosal
    • VMCM
    • VMCM1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab119559.



ab119559 has not yet been referenced specifically in any publications.

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