Overview

  • Product name
    Human TIMP1 ELISA Kit
    See all TIMP1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 40 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.49 84% - 104%
    Serum 92.43 81% - 102%
    Plasma 93.67 83% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TIMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP1 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human TIMP1 ELISA Kit (ab187394) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100651

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100651

Protocols

References

This product has been referenced in:
  • Wang QW  et al. Anti-influenza A virus activity of rhein through regulating oxidative stress, TLR4, Akt, MAPK, and NF-?B signal pathways. PLoS One 13:e0191793 (2018). Read more (PubMed: 29385192) »
  • Natrajan MS  et al. Pioglitazone regulates myelin phagocytosis and multiple sclerosis monocytes. Ann Clin Transl Neurol 2:1071-84 (2015). ELISA ; Human . Read more (PubMed: 26734659) »

See all 3 Publications for this product

Publishing research using ab100651? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
Votes: Highest First

Could you confirm that your Human TIMP-1 ELISA kit (ab100651) is in a form of a 8 wells of 12x strips instead of 1 whole 96 well plate please?

Thanks

Thank you for contacting us.

You are correct!, this kit comes with 12 strips of 8 wells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Dear technical team,

Our customer purchased
ab100586(lot.GR15494),
ab100662(lot.GR41054),
ab100606(lot.37486),
ab100610(lot.GR39199),
ab100653(lot.GR37277) and
ab100651(lot.GR37276)
last year.

Sh...

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Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very ...

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LOT NUMBER GR37276-1 DESCRIPTION OF THE PROBLEM Problem with standard curve SAMPLE serum PRIMARY ANTIBODY manufacturer DETECTION METHOD as per kit POSITIVE AND NEGATIVE CONTROLS USED n/a ANTIBODY STORAGE CONDITIONS 4 degrees celcius TYPE OF ELISA sand...

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Thank you for your inquiry and for forwarding your testing data. There are a few things I noticed after looking at the data: 1) The first thing I noticed was the background is quite high, ~0.5 OD. This high background is really reducing the dynamic ran...

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Does the kit detect free TIMP1 only or total TIMP1 (free + complex-bound + truncated)?

Thank you for contacting us. This kit ab100651 will detect free TIMP-1, both pro and active form (thus, total) however it is not known whether it will also detect the bound form (i.e. TIMP-MMP complexes).   I hope this information is helpful to you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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