Overview

  • Product name

    Human TIMP3 ELISA Kit (MIG-5)
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Saliva, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    625 pg/ml - 10000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human TIMP3 (MIG-5) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human TIMP3 (MIG-5) in cell culture supernatants, serum plasma (heparin, EDTA) and saliva.

    A TIMP3 (MIG-5) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for TIMP3 (MIG-5) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human TIMP3 (MIG-5) amount of sample captured in plate.

  • Notes

    Note: In our lab we were only able to detect high doses of TIMP3 above 625 pg/ml.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human TIMP3 antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human TIMP3 antibody 1 x 130µl
    Lyophilized recombinant Human TIMP3 standard 2 x 10ng
    Plate Seal 4 units
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Amber Bottle 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
  • Involvement in disease

    Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.
  • Sequence similarities

    Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • HSMRK222
    • K222
    • K222TA2
    • Metalloproteinase inhibitor 3
    • MIG 5 protein
    • Protein MIG-5
    • SFD
    • TIMP metallopeptidase inhibitor 3
    • TIMP-3
    • TIMP3
    • TIMP3_HUMAN
    • Tissue inhibitor of metalloproteinases 3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • TIMP3 measured in culture supernatants (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab119608.

Protocols

References

This product has been referenced in:

See all 4 Publications for this product

Customer reviews and Q&As

Question
Answer

We do not have in-house data for plasma but the reference listed on the datasheet used this TIMP3 ELISA to assay plasma from children. The levels in normal children averaged 1230pg/mL. So, to fall within the range of the standards, the samples were either not diluted at all or were at most diluted 1/2.

Here is a link to the reference. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3708036/

Cheng KS et al. Circulating matrix metalloproteinase-2 and -9 enzyme activities in the children with ventricular septal defect. Int J Biol Sci 9:557-63 (2013).

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