Overview

  • Product name

    Human TIMP4 ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 25 pg/ml
  • Range

    28.67 pg/ml - 7000 pg/ml
  • Recovery

    80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.11 89% - 104%
    Serum 73.49 67% - 82%
    Plasma 72.83 67% - 81%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TIMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP4 in serum, plasma (collect plasma using EDTA and citrate as an anticoagulant. Heparin is not recommended), cell culture supernatants.

    This assay employs an antibody specific for Human TIMP4 coated on a 96- well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab113328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113328

  • Representative Standard Curve using ab113328

Protocols

References

ab113328 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry. Unofrtunately there is no straightforward way to categorize which kits can be used with heparin and which cannot. You need to look at the datasheet or contact us regarding the specific kit, as different manufacturers and reagents may or may not be suitable with heparin.

The reason for not using heparin is fairly straightforward though. When we tested heparin plasma, the detection of TIMP-4 was significantly decreased by some sort of interference cause by the heparin. EDTA and citrate plasma had no such interference.

Please let me know if you have any further questions.

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