Key features and details
- Sensitivity: 25 pg/ml
- Range: 28.67 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman TIMP4 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 25 pg/ml
Range28.67 pg/ml - 7000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.11 89% - 104% Serum 73.49 67% - 82% Plasma 72.83 67% - 81%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s TIMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP4 in serum, plasma (collect plasma using EDTA and citrate as an anticoagulant. Heparin is not recommended), cell culture supernatants.
This assay employs an antibody specific for Human TIMP4 coated on a 96- well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human TIMP4 2 vials Recombinant Human TIMP4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP4 Microplate (12 strips x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml
- Metalloproteinase inhibitor 4
- TIMP 4
- TIMP metallopeptidase inhibitor 4
ab113328 has not yet been referenced specifically in any publications.