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My customer wants to do his research on cellular lysates with this test, but in the datasheet it is not written how much cells is needed for this test.
Asked on Jun 08 2017
The number of cells used per sample with ab108903 Human Tissue Factor ELISA Kit (CD142) will very much depend on the amount of TF in the specific sample being tested. If the expression level is low, you might want to try 1 million cell per sample (per measurement). If level is expected to be high, you can use much less. This will need to be individually optimized by customers for their own experiments.
I am sorry It is difficult to recommend how many cells to use per sample without further details of the samples being used. But I hope this suggestion will be helpful and a good starting point.
Answered on Jun 08 2017