Overview

  • Product name

    Human Tissue Factor Pathway Inhibitor ELISA Kit
    See all Tissue Factor Pathway Inhibitor kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    14.9 pg/ml
  • Range

    78.1 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 117 92% - 123%
    Serum 105 92% - 118%
    Cell culture media 97 95% - 99%
    Heparin Plasma 101 64% - 111%
    EDTA Plasma 112 100% - 125%
    Citrate Plasma 111 74% - 140%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TFPI protein in Human serum, plasma, urine and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Tissue factor pathway inhibitor (TFPI), also known as extrinsic pathway inhibitor (EPI) or Lipoprotein-associated coagulation inhibitor (LACI), inhibits coagulation through the formation of a complex with coagulation factor X, VII, and tissue factor (TF). This complex acts to prevent the activation of factor X which would lead to clot formation. This active 40 kDa full-length protein is found 50% in LDL, 40% in HLDL, and 10% in VLDL. Abnormally high levels of TFPI can lead to severe hemostatic disturbances such as thrombosis, disseminated intravascular coagulation, and septic shock. Similarly, TFPI is also used clinically to treat inflammatory and cardiovascular diseases where clot formation is a high prevalence.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human TFPI Capture Antibody 1 x 600µl
    10X Human TFPI Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    Human TFPI Lyophilized Recombinant Protein 2 vials
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • Tissue specificity

    Mostly in endothelial cells.
  • Sequence similarities

    Contains 3 BPTI/Kunitz inhibitor domains.
  • Domain

    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Secreted and Microsome membrane.
  • Information by UniProt
  • Alternative names

    • EPI
    • Extrinsic pathway inhibitor
    • LACI
    • Lipoprotein-associated coagulation inhibitor
    • TFPI
    • TFPI1_HUMAN
    • Tissue factor pathway inhibitor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab188392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (40 fold). The mean level of TFPI is 34,904 pg/mL with a range of 23,234 – 46,865 pg/mL and a standard deviation of 8,209 pg/mL.

Protocols

References

ab188392 has not yet been referenced specifically in any publications.

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