Overview

  • Product name

    Human TL1A ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich
  • Sensitivity

    15 pg/ml
  • Range

    39 pg/ml - 2500 pg/ml
  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's Human TL1A ELISA Kit (ab204532) is an ELISA kit for use with serum and cell supernatants. (Genecards: TNFRSF6B)

  • Notes

    TL1A is a ligand belonging to the tumor necrosis factor (TNF) family (also called TNFSF15). It is expressed predominantly by endothelial cells and monocytes. TL1A is upregulated by the proinflammatory cytokines TNFα and IL-1ß and also by immune complexes (IC). TL1A functions in T cell costimulation and Th1 polarization. On activated T cells, TL1A functions specifically via its surface-bound receptor DR3, (a member of the deathdomain containing TNF receptor family) to promote cell survival and secretion of proinflammatory cytokines. The secreted decoy receptor 3 (DcR3), a soluble protein of the tumor necrosis factor receptor (TNFR) superfamily blocks the action of TL1A. TL1A can be released to circulate as a homotrimeric soluble form.

    Activation of DR3 by TL1A induces the formation of a signaling complex containing TRADD, TRAF2, and RIP and activates the NF-?B and the ERK, JNK, and p38 mitogen-activated protein kinase pathways. The TL1A/DR3 pathway plays an important role in Th1-mediated intestinal diseases, such as Crohn’s disease. TL1A expression is increased in inflammatory bowel disease (IBD) and levels of TL1A protein as well as the number of TL1A-expressing cells correlate with the severity of inflammation, most significantly in Crohn's Disease.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    Assay Buffer 13, 10X Concentrate 1 x 30ml
    Plate Sealer 3 units
    Stop Solution (1N Soln.) 1 x 10ml
    Streptavidin-HRP 1 x 12.5µg
    TL1A Clear Microtiter Plate 1 unit
    TL1A Detection Antibody 1 x 20µl
    TL1A Standard 1 x 2µg
    TMB Substrate 1 x 10ml
    Wash Buffer Concentrate 1 x 28ml
  • Research areas

  • Function

    Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
  • Tissue specificity

    Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Secreted and Membrane.
  • Information by UniProt
  • Alternative names

    • MGC129934
    • MGC129935
    • tl1
    • TL1 A
    • tl1a
    • tnf ligand related molecule 1
    • TNF ligand-related molecule 1
    • tnf superfamily ligand tl1a
    • TNF15_HUMAN
    • TNFSF15
    • Tumor necrosis factor (ligand) superfamily member 15
    • Tumor necrosis factor ligand superfamily member 15
    • Tumor necrosis factor ligand superfamily member 15, secreted form
    • Vascular endothelial cell growth inhibitor
    • Vascular endothelial growth inhibitor
    • Vascular endothelial growth inhibitor 192a
    • vegi
    • VEGI192A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab204533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • The graph shown is for illustration only and should not be used to calculate results from another assay.

Protocols

References

ab204533 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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