Overview

  • Product name

    Human TLR2 ELISA Kit
    See all TLR2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Milk 5 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Milk 3 9.8%
  • Sample type

    Milk, Urine, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    17 pg/ml
  • Range

    109.4 pg/ml - 7000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 90 88% - 94%
    Urine 87 85% - 92%
    Serum 87 84% - 90%
    Cell Lysate 100 96% - 102%
    Cell culture media 102 97% - 108%
    Heparin Plasma 89 85% - 95%
    EDTA Plasma 97 96% - 100%
    Citrate Plasma 86 85% - 87%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Product overview

    TLR2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TLR2 protein in human serum, plasma, urine, milk, cell culture supernatant, cell and tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Toll-Like Receptor 2 (TLR2) is a transmembrane receptor expressed on a wide variety of cell types including microglia, monocytes, macrophages and dendritic cells. TLR2 cooperates with multiple other proteins (TLR1, LY96, and other) to mediate an immune response to bacterial lipoproteins. Cytokine secretion in response to bacteria is found to occur in a TLR2 dependent fashion.


    Sensitivity:


    Samples diluted in Sample Diluent NS + Enhancer - 74 pg/ml


    Samples diluted in 1X Cecll Extraction Buffer PTR - 17 pg/ml

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    10X Human TLR2 Capture Antibody 1 x 600µl
    10X Human TLR2 Detector Antibody 1 x 600µl
    Human TLR2 Lyophilized Recombinant Protein 1 x 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • Tissue specificity

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • Post-translational
    modifications

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • CD282
    • CD282 antigen
    • TIL 4
    • TIL4
    • TLR 2
    • TLR2
    • TLR2_HUMAN
    • Toll like receptor 2
    • Toll like receptor 2 precursor
    • Toll-like receptor 2
    • Toll/interleukin 1 receptor like 4
    • Toll/interleukin 1 receptor like protein 4
    • Toll/interleukin receptor like protein 4
    • Toll/interleukin-1 receptor-like protein 4
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab227897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between Human TLR2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 41.3 ng/mL in milk.

  • The concentrations of TLR2 were measured in duplicate and interpolated from the TLR2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 2.3 ng/mL in unstimulated THP-1 and 6.0 ng/mL in stimulated THP-1.

Protocols

References

ab227897 has not yet been referenced specifically in any publications.

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