Human TLR2 ELISA Kit (ab227897)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17 pg/ml
- Range: 109.4 pg/ml - 7000 pg/ml
- Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TLR2 ELISA Kit
See all TLR2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Milk 5 3.1% Inter-assay Sample n Mean SD CV% Milk 3 9.8% -
Sample type
Milk, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17 pg/ml -
Range
109.4 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 90 88% - 94% Urine 87 85% - 92% Serum 87 84% - 90% Cell Lysate 100 96% - 102% Cell culture media 102 97% - 108% Hep Plasma 89 85% - 95% EDTA Plasma 97 96% - 100% Cit plasma 86 85% - 87% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TLR2 ELISA Kit (ab227897) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TLR2 protein in cit plasma, edta plasma, hep plasma, milk, serum, urine, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TLR2 with 17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
The standard is affinity purified.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TLR2 Capture Antibody 1 x 600µl 10X Human TLR2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human TLR2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. -
Tissue specificity
Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. -
Sequence similarities
Belongs to the Toll-like receptor family.
Contains 14 LRR (leucine-rich) repeats.
Contains 1 TIR domain. -
Post-translational
modificationsGlycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD282
- CD282 antigen
- TIL 4
see all -
Database links
- Entrez Gene: 7097 Human
- Omim: 603028 Human
- SwissProt: O60603 Human
- Unigene: 519033 Human
Associated products
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Alternative Versions
Images
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Other - Human TLR2 ELISA Kit (ab227897)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human TLR2 Competitor standard curve comparisonStandard curve comparison between Human TLR2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of Human TLR2 standard curve in Sample Diluent NS+ EnhancerBackground-subtracted data values (mean +/- SD) are graphed.
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Example of Human TLR2 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked TLR2 in Human serum, and plasma samplesThe concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spiked TLR2 in Human cell culture media and urine samplesThe concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50% and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TLR2 in Human milk sampleThe concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 41.3 ng/mL in milk.
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Interpolated concentrations of native TLR2 in Human unstimulated THP-1 and stimulated THP-1 based on a 200 µg/mL extract loadThe concentrations of TLR2 were measured in duplicate and interpolated from the TLR2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 2.3 ng/mL in unstimulated THP-1 and 6.0 ng/mL in stimulated THP-1.
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Sandwich ELISA - Human TLR2 ELISA Kit (ab227897)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227897 has been referenced in 1 publication.
- Yang Y et al. Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kßsignaling. J Reprod Immunol 142:103192 (2020). PubMed: 32950783