Key features and details
- Suitable for: Blocking
Product nameHuman TMEM16A peptide
Purity70 - 90% by HPLC.
Our Abpromise guarantee covers the use of ab97423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-TMEM16A antibody (ab84915)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
- ANO 1
FunctionActs as a calcium-activated chloride channel. Required for normal tracheal development.
Tissue specificityBroadly expressed with higher levels in liver and skeletal muscle.
Sequence similaritiesBelongs to the anoctamin family.
DomainThe region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.
Cellular localizationCell membrane. Cytoplasm.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97423 has been referenced in 1 publication.
- Crutzen R et al. Anoctamin 1 (Ano1) is required for glucose-induced membrane potential oscillations and insulin secretion by murine ß-cells. Pflugers Arch 468:573-91 (2016). PubMed: 26582426