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|Sample type||Average %||Range|
|Serum||81||74% - 90%|
Abcam’s TNF alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant Human TNF alpha.
Get results in 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Biotinylated anti-TNF alpha||Red||1 x 400µl||2 x 400µl|
|TMB Substrate||1 x 11ml||1 x 24ml|
|TNF alpha Control||Yellow||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Plastic Plate Covers||1 x 2 units||1 x 4 units|
|Standard Diluent (Human Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
|TNF alpha Microplate (12 x 8 well strips)||1 unit||2 units|
|TNF alpha Standard||2 vials||4 vials|
Our Abpromise guarantee covers the use of ab46087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative standard curve using ab46087
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"