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Abcam’s TNFα Human in vitro ELISA Set is designed for the quantitative measurement of TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The TNF alpha Elipair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This Elipair set is intended to measure human TNF alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant human TNF alpha.
|Components||5 x 96 tests||10 x 96 tests|
|Detection Biotinylated anti-TNF alpha Antibody||1 x 0.55ml||2 x 0.55ml|
|TMB Substrate||2 vials||4 vials|
|Streptavidin-HRP||1 vial||2 vials|
|TNF alpha Capture Antibody||1 x 0.5ml||2 x 0.5ml|
|TNF alpha Standard||5 vials||10 vials|
Our Abpromise guarantee covers the use of ab47347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab47347 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"