Recombinant

Human TNF alpha Matched Antibody Pair Kit (ab213467)

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Human TNF-alpha standard curve.
  • Sandwich ELISA - Human TNF alpha Matched Antibody Pair Kit (ab213467)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 1.95 pg/ml - 1000 pg/ml

Overview

  • Product name

    Human TNF alpha Matched Antibody Pair Kit
    See all TNF alpha kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    1.18 pg/ml

  • Range

    1.95 pg/ml - 1000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human TNF alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human TNF alpha.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab181421. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISA, IAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 5 x 96 tests
    Human TNF alpha Capture Antibody 1 x 50µg
    Human TNF alpha Detector Antibody 1 x 12.5µg
    Human TNF alpha Lyophilized Protein 1 vial

  • Research areas

  • Function

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

  • Involvement in disease

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Alternative names

    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily member 2
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor (TNF superfamily member 2)
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all

  • Database links

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab213467 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    IA
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    IA
    Use at an assay dependent concentration.

    Images

    • Human TNF-alpha standard curve.
      Human TNF-alpha standard curve.
    • Sandwich ELISA - Human TNF alpha Matched Antibody Pair Kit (ab213467)
      Sandwich ELISA - Human TNF alpha Matched Antibody Pair Kit (ab213467)
      To learn more about the advantages of recombinant antibodies see here.

    References (1)

    Publishing research using ab213467? Please let us know so that we can cite the reference in this datasheet.

    ab213467 has been referenced in 1 publication.

    • Bari SMI  et al. Calorimetric sandwich-type immunosensor for quantification of TNF-a. Biosens Bioelectron 126:82-87 (2019). PubMed: 30396021

    Customer reviews and Q&As

    Show All Reviews Q&A
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    ELISA good

     Excellent Good 4/5 (Ease of Use)
    Abreviews
    abreview image
    The ELISA test was conducted, and the sensitivity was excellent, and the result was stable even when the general protocol given by Abcam was used for detection.

    Abcam user community

    Verified customer

    Submitted May 12 2021

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