Overview

  • Product name

    Human TNF beta ELISA Kit
    See all LTA kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.2%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.6 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    105 %

    Sample specific recovery
    Sample type Average % Range
    Serum 105 87% - 130%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s TNF beta Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TNF beta concentrations in cell culture supernatant, serum and plasma.

    TNF beta specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with HRP-conjugated TNF beta detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is the added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the TNF beta amount of sample captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 2 units
    HRP-Conjugate anti-human TNF beta monoclonal antibody 1 x 200µl
    Microplate coated with monoclonal antibody to Human TNF beta (12 x 8 wells) 1 unit
    Sample Diluent 1 x 5ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
    TNF beta Standard 2 x 50µl
  • Research areas

  • Function

    Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • Involvement in disease

    Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
  • Information by UniProt
  • Alternative names

    • LTalpha
    • DIF
    • LT
    • LT alpha
    • LT-alpha
    • Lta
    • Lymphotoxin alpha
    • Lymphotoxin alpha (TNF superfamily, member 1)
    • Lymphotoxin-alpha
    • TNF B
    • TNF superfamily member 1
    • TNF, lymphocyte-derived
    • TNF-beta
    • TNFB
    • TNFB_HUMAN
    • TNFbeta
    • TNFSF1
    • TNLG1E
    • Tumor necrosis factor beta
    • tumor necrosis factor ligand 1E
    • Tumor necrosis factor ligand superfamily member 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119561.

Protocols

References

ab119561 has not yet been referenced specifically in any publications.

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