Overview

  • Product name
    Human TNFRSF18 ELISA Kit (GITR)
    See all TNFRSF18 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    13.7 pg/ml - 10000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.69 84% - 105%
    Serum 94.18 83% - 104%
    Plasma 92.75 82% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TNFRSF18 2 vials
    Recombinant Human TNFRSF18 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
    TNFRSF18 Microplate (12 x 8 wells) 1 unit
  • Research areas
  • Relevance
    TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.
  • Alternative names
    • Activation inducible TNFR family receptor
    • AITR
    • GITR
    • GITR D
    • Glucocorticoid induced TNFR related protein
    • Tumor necrosis factor receptor superfamily member 18
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab100527 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit
      ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit

      GITR measured in PBMC lysates (RIPA; 0.02-0.1 mg of proteins tested per well) after 72 hours of culture (1e6 cells per mL) in 0.1% DMSA (CTRL), 5 ug x mL-1 PHA (Sigma) or in 50ng x mL-1 PMA (ab120297) with 1uM ionomycin (ab120116) (P/I) (duplicates +/- SD).

    • ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit
      ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit

      GITR measured in undiluted human biological fluids (undiluted; duplicates +/- SD).

    • ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit
      ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit

      Standard curve in diluent B, with background signal subtracted (duplicates; +/- SD).

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100527

    • ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit
      ELISA: ab100527 TNFRSF18 (GITR) Human ELISA Kit

      Standard curve in diluent A, with background signal subtracted (duplicates; +/- SD).

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100527

    Protocols

    References

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