Overview

  • Product name

    Human TNFSF4 ELISA Kit (OX40L)
    See all CD252 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.96ng/ml 0.067 3.4%
    2 16 3.17ng/ml 0.143 4.5%
    3 16 5.86ng/ml 0.299 5.1%
    Inter-assay
    Sample n Mean SD CV%
    1 24 2.07ng/ml 0.087 4.2%
    2 24 3.24ng/ml 0.188 5.8%
    3 24 6.15ng/ml 0.381 6.2%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human TNFSF4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (OX40L) (ab213842) is designed for the quantitative measurement of Human TNFSF4 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF4/OX40L has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q51-L183) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF4/OX40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFSF4/OX40L amount of sample captured in plate.

  • Notes

    OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human TNFSF4 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-Human TNFSF4 antibody 1 x 130µl
    Lyophilized recombinant Human TNFSF4 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
  • Involvement in disease

    Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • CD 134L
    • CD 252
    • CD134
    • CD134 ligand
    • CD134L
    • CD252
    • CD252 antigen
    • glycoprotein 34 kd
    • Glycoprotein Gp34
    • GP34
    • OX-40L
    • OX40 antigen ligand
    • OX40 ligand
    • OX40L
    • TAX transcriptionally-activated glycoprotein 1
    • TNFL4_HUMAN
    • Tnfsf4
    • Tumor necrosis factor (ligand) superfamily member 4
    • Tumor necrosis factor ligand superfamily member 4
    • TXGP1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human TNFSF4 ELISA Kit (OX40L) (ab213842) Standard Curve.

Protocols

References

This product has been referenced in:

  • Ma SL & Zhang L Elevated serum OX40L is a biomarker for identifying corticosteroid resistance in pediatric asthmatic patients. BMC Pulm Med 19:66 (2019). Read more (PubMed: 30890137) »
  • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). Read more (PubMed: 30773478) »
See all 2 Publications for this product

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