Product nameHuman TOP3B peptide
See all TOP3B proteins and peptides
Our Abpromise guarantee covers the use of ab199552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
- DNA topoisomerase 3 beta 1
- DNA topoisomerase 3-beta-1
- DNA topoisomerase III beta 1
FunctionPossesses negatively supercoiled DNA relaxing activity.
Tissue specificityIsoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
Sequence similaritiesBelongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain.
- Information by UniProt
ab199552 has not yet been referenced specifically in any publications.