Key features and details
- Suitable for: Blocking
Product nameHuman TOP3B peptide
Our Abpromise guarantee covers the use of ab199552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
- DNA topoisomerase 3 beta 1
- DNA topoisomerase 3-beta-1
- DNA topoisomerase III beta 1
FunctionPossesses negatively supercoiled DNA relaxing activity.
Tissue specificityIsoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
Sequence similaritiesBelongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab199552 has not yet been referenced specifically in any publications.