Human Total Cholesterol ELISA Kit (ab287836)
Key features and details
- Sensitivity: 0.1 mM
- Range: 1.25 mM - 20 mM
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Other biological fluids, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Human
Overview
-
Product name
Human Total Cholesterol ELISA Kit
See all Cholesterol kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Urine, Serum, Other biological fluids, EDTA Plasma, Cit plasma -
Assay type
Sandwich -
Sensitivity
< 0.1 mM -
Range
1.25 mM - 20 mM -
Assay time
1h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Total Cholesterol ELISA Kit (ab287836), previously known as QuickDetect Total Cholesterol (Human) ELISA Kit (Cat# K4431-100), is a sandwich ELISA assay for measuring the quantity of human total cholesterol in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes, with fewer incubation and wash steps than other kits, resulting in less errors and more consistent results.
Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Store entire kit in the dark.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash buffer 1 x 25ml Chomogen Solution A 1 x 6ml Chomogen Solution B 1 x 6ml HRP-Conjugate Reagent 1 x 10ml Micro ELISA strip-plate 1 unit Plate Sealer 2 units Sample Diluent 1 x 6ml Standard (40 mmol/L) 1 x 500µl Standard Diluent 1 x 6ml Stop Solution 1 x 6ml -
Research areas
-
Relevance
Cholesterol is a waxy substance that occurs naturally in all parts of the body and that the body needs to function normally. It is present in cell walls or membranes everywhere in the body, including the brain, nerves, muscle, skin, liver, intestines, and heart. The body uses cholesterol to produce many hormones, vitamin D, and the bile acids that help to digest fat. It takes only a small amount of cholesterol in the blood to meet these needs. However, if you have too much cholesterol in your bloodstream, it can lead to atherosclerosis, a condition in which fat and cholesterol are deposited in the walls of the arteries in many parts of the body, including the coronary arteries feeding the heart. In time, narrowing of the coronary arteries by atherosclerosis can produce the signs and symptoms of heart disease, including angina and heart attack.
Images
-
Example data
Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab287836 has not yet been referenced specifically in any publications.