Overview

  • Product name

    Human Total Prostate Specific Antigen ELISA Kit
    See all Prostate Specific Antigen kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Urine 8 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Urine 3 4.5%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    9.4 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 101 98% - 104%
    Serum 84 80% - 87%
    Cell culture media 91 91% - 92%
    Heparin Plasma 83 75% - 91%
    EDTA Plasma 87 82% - 94%
    Citrate Plasma 87 84% - 94%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Goat, Guinea pig, Hamster, Cow, Dog
  • Product overview

    Total Prostate Specific Antigen (total PSA) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Total PSA protein in human serum, plasma, urine and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab188388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 3) are graphed.

  • Mean Human male Total PSA is 424 pg/mL and values fall within expected normal reference ranges (WHO). Observed Free PSA levels were also determined using the Free Prostate Specific Antigen (free PSA) SimpleStep ELISA ab188389. Mean Human male Free PSA is 50.2 pg/mL or 11% Free PSA and values fall within expected normal reference ranges (WHO).

  • Mean Human female Total PSA is 12.5 pg/mL (50% detectable) and values fall within expected normal reference ranges (WHO). Observed Free PSA levels were also determined using the Free Prostate Specific Antigen (free PSA) SimpleStep ELISA ab188389. Mean Human female Free PSA is 12.7 pg/mL and values fall within expected normal reference ranges (WHO).

  • Mean Human Total PSA values fall within expected normal reference ranges (WHO). Observed Free PSA levels were also determined using the Free Prostate Specific Antigen (free PSA) SimpleStep ELISA ab188389. Mean Human Free PSA values fall within expected normal reference ranges (WHO). Please note: overall Total and Free PSA levels are lower due to mixed gender samples.

  • Mean Human Total PSA values are 71.7 ng/mL and fall within expected normal reference ranges (WHO). Observed Free PSA levels were also determined using the Free Prostate Specific Antigen (free PSA) SimpleStep ELISA ab188389. Mean Human Free PSA is 61.9 ng/mL and values fall within expected normal reference ranges (WHO).

  • Mean Human Total PSA is 0.54 ng/mL in normal serum and values fall within expected normal reference ranges (WHO). Observed Total PSA in each prostate cancer donor; Donor 76, 48 ng/mL; Donor 77, 120 ng/mL; Donor 78, 40 ng/mL. Observed Free PSA levels were also determined using the Free Prostate Specific Antigen (free PSA) SimpleStep ELISA ab188389. Mean Human Free PSA is 0.03 ng/mL (5% Free PSA) and values fall within expected normal reference ranges (WHO). Observed Free PSA in each prostate cancer donor; Donor 76, 9.2 ng/mL (19.2% Free PSA); Donor 77, 49 ng/mL (40.6% Free PSA); Donor 78, 7 ng/mL (17.1% Free PSA). The significant increase observed in Total and Free PSA (including % Free PSA) correlates well with the diagnosis of Prostate Cancer.

Protocols

References

This product has been referenced in:

  • Hussain SS  et al. Suppression of ribosomal protein RPS6KB1 by Nexrutine increases sensitivity of prostate tumors to radiation. Cancer Lett 433:232-241 (2018). Read more (PubMed: 30003927) »
  • Solivio MJ  et al. Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera. Sci Rep 6:24186 (2016). Read more (PubMed: 27068126) »
See all 2 Publications for this product

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