Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Toxocara canis IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos Serum 12 2.6% Inter-assay Sample n Mean SD CV% Pos. Serum 5 8.1%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Toxocara canis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Toxocara canis in Human serum and plasma.
A 96-well plate has been precoated with Toxocara canis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Toxocara canis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxocara canis IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Toxocara canis Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Toxocara canis Cut-off Control Yellow, Green cap 1 x 3ml Toxocara canis Negative Control Yellow, Blue cap 1 x 2ml Toxocara canis Positive Control Yellow, Red cap 1 x 2ml Toxocara canis Protein A HRP Conjugate Blue, Black cap 1 x 20ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108775 has been referenced in 1 publication.
- Phasuk N & Punsawad C Seroprevalence of Toxocara canis infection and associated risk factors among primary schoolchildren in rural Southern Thailand. Trop Med Health 48:23 (2020). PubMed: 32336929