Human Anti-Toxoplasma gondii IgG ELISA Kit (ab108776)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameHuman Anti-Toxoplasma gondii IgG ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Standard B 6 5.7% Standard C 6 5.3% Standard D 6 6.2% Inter-assay Sample n Mean SD CV% Standard B 4 7.1% Standard C 4 10.1% Standard D 4 6.3% -
Sample typeSerum, Heparin Plasma, Citrate Plasma
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Assay typeIndirect
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Sensitivity< 1 IU/ml
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Range1 U/ml - 200 U/ml
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s anti-Toxoplasma gondii IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.
A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.
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Tested applicationsSuitable for: Indirect ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Toxoplasma gondii (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Toxoplasma gondii anti-IgG HRP Conjugate Black cap 1 x 20ml Toxoplasma gondii IgG Standard A – 0 U/mL Yellow, Blue cap 1 x 2ml Toxoplasma gondii IgG Standard B – 50 U/mL Yellow, Green Cap 1 x 2ml Toxoplasma gondii IgG Standard C – 100 U/mL Yellow, Yellow cap 1 x 2ml Toxoplasma gondii IgG Standard D – 200 U/mL Yellow, Red Cap 1 x 2ml -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab108776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | Use at an assay dependent dilution. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab108776
Protocols
References
ab108776 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"