Human Anti-Toxoplasma gondii IgG ELISA Kit (ab108776)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 1 IU/ml
    • Range: 1 U/ml - 200 U/ml
    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

    Overview

    • Product name

      Human Anti-Toxoplasma gondii IgG ELISA Kit
      See all Toxoplasma gondii IgG kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Standard B 6 5.7%
      Standard C 6 5.3%
      Standard D 6 6.2%
      Inter-assay
      Sample n Mean SD CV%
      Standard B 4 7.1%
      Standard C 4 10.1%
      Standard D 4 6.3%

    • Sample type

      Serum, Hep Plasma, Cit plasma

    • Assay type

      Indirect

    • Sensitivity

      < 1 IU/ml

    • Range

      1 U/ml - 200 U/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s anti-Toxoplasma gondii IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.


      A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.

    • Platform

      Microplate

    Properties

    Associated products

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108776

    References (1)

    Publishing research using ab108776? Please let us know so that we can cite the reference in this datasheet.

    ab108776 has been referenced in 1 publication.

    • Park YT  et al. Soft Tissue Augmentation with Silk Composite Graft. Maxillofac Plast Reconstr Surg 36:192-200 (2014). PubMed: 27489833

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    We would be interested in obtaining a quote for the ToRCH range of assays. In particular:


    Toxoplasma IgG

    Toxoplasma IgM

    Rubella IgG

    Rubella IgM

    CMV IgG

    CMV IgM

    HSV-1 IgG

    HSV-2 IgG

    HSV-1 & 2 IgM


    We look forward to hearing from you soon.

    Kind regards,

    Read More
    Answer

    Thank you for your email.

    The product I have identified for you are as follows;

    ab108776
    ab108778

    ab108767
    we do not have anti rubella IgM kit

    ab108724
    ab108725

    ab108737
    ab108738

    ab108739
    ab108740

    ab108742

    I have contacted my colleague who will give you a bulk quote for this kit. Please let me know if you do not hear from us within 24 hours of this email.

    I hope this information will be helpful. Should you require more information please do not hesitate to contact me.

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    Question

    Phoned to inquire about the immunogen used to coat the plates of this product. Is this base from the tachyzoites or from the oocyst. They also inquired as to whether the Standard A should have the same reading as the blank or if it was generally higher.

    Read More
    Answer

    Thank you for contacting us.

    In regards to the Toxoplasma gondii IgG ELISA, ab108776, the wells in this kit are coated with tachyzoites.

    I also asked about the expected reads for Standard A in comparison with the blank. In this application the Standard A read should be almost as low as the blank.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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