Human Anti-Toxoplasma gondii IgG ELISA Kit (ab108776)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human Anti-Toxoplasma gondii IgG ELISA Kit
See all Toxoplasma gondii IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Standard B 6 5.7% Standard C 6 5.3% Standard D 6 6.2% Inter-assay Sample n Mean SD CV% Standard B 4 7.1% Standard C 4 10.1% Standard D 4 6.3% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
< 1 IU/ml -
Range
1 U/ml - 200 U/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Toxoplasma gondii IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.
A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.
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Tested applications
Suitable for: Indirect ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Toxoplasma gondii (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Toxoplasma gondii anti-IgG HRP Conjugate Black cap 1 x 20ml Toxoplasma gondii IgG Standard A – 0 U/mL Yellow, Blue cap 1 x 2ml Toxoplasma gondii IgG Standard B – 50 U/mL Yellow, Green Cap 1 x 2ml Toxoplasma gondii IgG Standard C – 100 U/mL Yellow, Yellow cap 1 x 2ml Toxoplasma gondii IgG Standard D – 200 U/mL Yellow, Red Cap 1 x 2ml -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab108776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | Use at an assay dependent dilution. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab108776
Protocols
References
This product has been referenced in:
- Park YT et al. Soft Tissue Augmentation with Silk Composite Graft. Maxillofac Plast Reconstr Surg 36:192-200 (2014). Read more (PubMed: 27489833) »
