Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Product nameHuman Anti-Toxoplasma gondii IgM ELISA Kit
Intra-assay Sample n Mean SD CV% Serum 1 24 2.9% Serum 2 24 1.5% Inter-assay Sample n Mean SD CV% Serum 2 12 6.5% Serum 3 12 4.5%
Sample typeSerum, Hep Plasma, Cit plasma
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
ab108778 Toxoplasma gondii IgM Human ELISA Kit is intended for the qualitative determination of IgM class antibodies against Toxoplasma gondii in human serum or plasma (citrate).
Toxoplasma gondii is a small intracellular parasite, whose live cycle has a sexual and an asexual phase. Sexual development is restricted to the intestinal cells of (probably exclusively) cats; the oocysts formed are excreted and due to their resistant cell walls they may be infectious under advantageous circumstances for at least 1 year. Animals and man are intermediate hosts for the asexual proliferation of T. gondii: the ingested parasites will proliferate explosively within the host cells lysing them eventually. They disseminate throughout the body via circulation and lymphatic system and may infect any cell type. In muscle and brain cells cysts are formed which are spheroidal and about 5-100 µm in diameter. Cysts are virtually immortal in the intermediate host.
Toxoplasma gondii is the most common parasite in humans, but its abundance (7-80%) is highly dependent on the geographic area, the socio-economic status and the nutritional customs. Infection only rarely causes toxoplasmosis and usually clinical symptoms are absent, but may produce severe problems in immunosuppressed persons and fetus.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Anti-Human (IgM) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Cover foil 1 unit IgM Sample Diluent Colored yellow 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Toxoplasma gondii HRP Conjugate 1 x 15ml Toxoplasma gondii IgM Cut-off Control Colored yellow; Green cap 1 x 3ml Toxoplasma gondii IgM Negative Control Colored yellow; Blue cap 1 x 2ml Toxoplasma gondii IgM Positive Control Colored yellow; Red cap 1 x 2ml
ab108778 has not yet been referenced specifically in any publications.