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Human TRAIL ELISA Kit (ab285340)

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Human TRAIL ELISA Kit

    Key features and details

    • Sensitivity: 9.375 pg/ml
    • Range: 15.6 pg/ml - 1000 pg/ml
    • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Homogenate
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)

    Overview

    • Product name

      Human TRAIL ELISA Kit
      See all TRAIL kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Homogenate

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 9.375 pg/ml

    • Range

      15.6 pg/ml - 1000 pg/ml

    • Assay duration

      Multiple steps standard assay

    • Product overview

      The Human TRAIL ELISA Kit (ab285340, K4262) is a sandwich ELISA assay for the quantitative measurement of human TNFSF10 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human TNFSF10 captured from the samples.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K4262 TRAIL/TNFSF10 (Human) ELISA Kit. K4262-100 is the same size as the 96 test size of ab285340.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 96 tests
      25X Wash buffer 1 x 30ml
      Antibody dilution buffer 1 x 10ml
      Biotin-Detection antibody (Concentrated) 1 x 120µl
      HRP-Streptavidin Conjugate (SABC) 1 x 120µl
      Lyophilized Standard 2 vials
      Micro ELISA Plate 1 unit
      Plate Sealer 5 units
      SABC dilution buffer 1 x 10ml
      Sample and Standard Dilution Buffer 1 x 20ml
      Stop Solution 1 x 10ml
      TMB Substrate 1 x 10ml

    • Research areas

    • Function

      Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

    • Tissue specificity

      Widespread; most predominant in spleen, lung and prostate.

    • Sequence similarities

      Belongs to the tumor necrosis factor family.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Alternative names

      • Apo 2 ligand
      • APO 2L
      • Apo-2 ligand
      • Apo-2L
      • APO2L
      • CD253
      • CD253 antigen
      • Chemokine tumor necrosis factor ligand superfamily member 10
      • Protein TRAIL
      • TL2
      • TNF Related Apoptosis Inducing Ligand
      • TNF related apoptosis inducing ligand TRAIL
      • TNF-related apoptosis-inducing ligand
      • TNF10_HUMAN
      • TNFSF10
      • TRAIL
      • Tumor necrosis factor (ligand) family member 10
      • Tumor Necrosis Factor (ligand) Superfamily Member 10
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta
      • Tumor necrosis factor ligand superfamily member 10
      see all

    Images

    • Human TRAIL ELISA Kit
      Human TRAIL ELISA Kit

      Standard curve for Human TRAIL ELISA Kit ab285340, A standard curve must be run with each assay.

    References (0)

    Publishing research using ab285340? Please let us know so that we can cite the reference in this datasheet.

    ab285340 has not yet been referenced specifically in any publications.

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